Determination of monosaccharide composition in polysaccharides of Coreopsis basalis by gas chromatography
CAO Zhilong1 and MA Xiaoli2. 1. Department of Pharmacy,Xinjiang Production and Construction Corps Hospital of Chinese People’s Armed Police Forces,Urumqi 830063,China；2. Instrumental Analysis Center,Xinjiang Medical University,Urumqi 830002,China
Abstract：Objective To establish a gas chromatographic method for determining the monosaccharide compositions and contents in polysaccharides of Coreopsis basalis. Methods Polysaccharides of Coreopsis basalis were derivatized after hydrolysis into monosaccharides with 4 mol/l TFA. Monosaccharide derivatives were separated on Shimadzu DM-5 column (30 m×0.25 mm，0.25 μm). The six monosaccharides were simultaneously separated under optimized conditions within 45 minutes. The calibration plots were linear over a range from 0.6 to 10 mg/ml. The recovery was 101.5%-103.4%. Results Polysaccharides of Coreopsis basalis consisted of Rha, Ara, Xyl, Man, Glu, and Gal with a molar ratio of 0.42∶2.51∶0.48∶0.83∶6.38∶2.07. Conclusions This method is simple, sensitive, accurate and reliable. It can be applied to quality control and determination of monosaccharide composition of Coreopsis basalis.
曹志龙,马晓丽. 气相色谱法测定新疆金鸡菊多糖中单糖的组成[J]. 武警医学, 2012, 23(10): 848-850.
CAO Zhilong and MA Xiaoli. . Department of Pharmacy,Xinjiang Production and Construction Corps Hospital of Chinese People’s Armed Police Forces,Urumqi,China；. Instrumental Analysis Center,Xinjiang Medical University,Urumqi,China. Determination of monosaccharide composition in polysaccharides of Coreopsis basalis by gas chromatography. Med. J. Chin. Peop. Armed Poli. Forc., 2012, 23(10): 848-850.
2012, Vol. 23 
