A study of resveratrol to suppress proliferation of human bladder cancer cell line T24 and to affect expression of miR-34a
WANG Wei1,LI Tan2,LI Ying1,LI Hui1
1.Department of Nephrology, The Affiliated Hospital of Logistics Institute of the Chinese People’s Armed Police Forces, Tianjin 300162; 2.Department of Immunology, Logistics Institute of the Chinese People’s Armed Police Forces, Tianjin 300162，China
Abstract：Objective To investigate the effects of resveratrol on proliferation of human bladder cancer cell line T24，and the role of miR-34a in its mechanism. Methods T24 cells were treated with resveratrol in different concentrations for 24 h. Cell proliferation was validated by MTT. Flow cytometry was used to analyse apoptosis. The expression of miR-34a was detect by real-time PCR. Results Resveratrol could inhibit the proliferation of T24 in a dose dependent manner, induce apoptosis and increase the expression of p53 significantly. Furthermore, the level of miR-34a in T24 cells was higher in resveratrol-treated group than that in the control group. Conclusions Resveratrol can suppress the proliferation of human bladder cancer cells, which may be attributed to inducing apoptosis, up-regulating miR-34a and p53.
王 伟,李 覃,李 瑛,李 辉. 白藜芦醇抑制膀胱癌细胞生长增殖及影响miR-34a表达的研究[J]. , 2012, 23(12): 1062-1064.
WANG Wei,LI Tan,LI Ying,LI Hui. A study of resveratrol to suppress proliferation of human bladder cancer cell line T24 and to affect expression of miR-34a. , 2012, 23(12): 1062-1064.
2012, Vol. 23 
