Abstract：Objective To investigate the effect of polyphyllin on the proliferation of osteoblast-like cells MC3T3-E1 and possible mechanisms. Methods The osteoblast-like cells MC3T3-E1, were administered with different concentrations of polyphyllin (3, 10, 30, and 100 μg/ml). Then, MTT colorimetric test, EdU kit, and alkaline phosphatase (ALP) activity detection kit were used to observe the proliferation of MC3T3-E1. Western blot analysis was employed to observe the expression of β-catenin and bone morphogenetic protein -2 (BMP-2) in MC3T3-E1 cells treated with 30, and 100 μg/ml of polyphyllin. Results Polyphyllin could effectively promote proliferation of MC3T3-E1 cells and upregulate the expression of β-catenin and BMP-2 expression. Conclusions Polyphyllin may promote MC3T3-E1 cell proliferation and differentiation via regulating β-catenin and BMP-2 signaling pathway.
许晓莲,李 勃. 重楼皂苷促成骨样细胞MC3T3-E1增殖作用及其机制[J]. , 2014, 25(3): 275-277.
XU Xiaolian and LI Bo. Effect of polyphyllins on proliferation of osteoblast-like cells MC3T3-E1 and its possible mechanisms. , 2014, 25(3): 275-277.
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2014, Vol. 25 
