Abstract：Objective Using Cerenkov luminescence imaging, making radionuclide optical molecular imaging of gastric cancer xenograft in nude mice, to study the feasibility of Cerenkov luminescence in diagnosis of gastric cancer. Methods Two gastric cancer xenografts in nude mice were injected in tail vein with 1.67× 107 Bq 18F-FDG and 3.34×107 Bq 18F-FDG, respectively. Cerenkov luminescence images were taken 1 hour after, then the same size ROI was selected for signal strength analysis. After imaging, the nude mice were killed. Images of xenograft, liver, kidney, heart, gastrointestinal tract, and lung were also taken. Then Cerenkov luminescence dynamic imaging was performed. 1 gastric cancer xenograft in nude mice was injected in tail vein with 1.67×107Bq 18F-FDG. Images were taken respectively in 40,60,80,100,120,140,160,180 min after injection, then compared. Results The signal strengths of xenograft were stronger. The signal strength relied on the activity of 18F-FDG in tail vein injection. The photons of tumor on gastric cancer xenografts injected with 3.34×107 Bq 18F-FDG and 1.67×107 Bq 18F-FDG were 8.432×105 p/s and 3.172×105 p/s, respectively. The signal strength gradually weakened over time. Conclusions Cerenkov luminescence imaging can achieve gastric cancer xenograft in nude mice, with the potential of diagnosing gastric cancer by optical molecular imaging.
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