Construction of a new tissue engineering skin detection model using HaCaT cells
LI Yan1,2, ZHOU Zhoulin1,3, LU Tao1,and LI Ruixin4
1.Department of Dermatology, The Armed Police Hospital of Tianjin, Tianjin 300162, China; 2..North China University of Science and Technology, Tangshan 063000, China; 3.Hospital of Chinese People’s Armed Police Force 8730 Army, Guangzhou 510800, China; 4.Academy of Military Medical Sciences, Tianjin 300161, China
Abstract：Objective To construct human immortalized keratinocytes HaCaT as seed cells for tissue engineering skin model.Methods A novel tissue engineered skin model was constructed for the detection of human immortalized keratinocytes and fibroblasts as seed cells. 10 kinds of chemicals were selected as test materials, the surface of local contact skin model was 15 min, and the relative viability of cells was measured by MTT colorimetry.Results Three different batches of the model, based on the relative values of cell viability as a decision, the new detection model constructed could determine eight kinds of harsh chemicals,after addition of IL-1α experiment improved the accuracy of the model, the accuracy rate was 75%.Conclusions This new model provides an effective in vitro test results and prove the feasibility of the model. At the same time it should use a greater number of substances for further validation and tests in different lab oratories to properly assess reproducibility.
李 燕，周宙霖，卢 涛，李瑞欣. 利用HaCaT细胞构建新型组织工程皮肤检测模型[J]. 武警医学, 2016, 27(7): 672-675.
LI Yan, ZHOU Zhoulin, LU Tao,and LI Ruixin. Construction of a new tissue engineering skin detection model using HaCaT cells. Med. J. Chin. Peop. Armed Poli. Forc., 2016, 27(7): 672-675.
Jírová D, Basketter D,Liebsch M, et al. Comparison of human skin irritation patch testdata with in vitro skin irritation assaysand animal data[J]. Contact Dermatitis ,2010, 62: 109-116.
[3]
Eskes C, Cole T, Hoffmann S, et al. The ECVAM International Validation Study on In VitroTests for Acute Skin Irritation: Selection of Test Chemicals[J]. Alternatives to Laboratory Animals, 2007,35(6):603-619.
Kandarova H, Liebsch M, Genschow E, et al. Optimisation of the EpiDerm test protocol for the upcoming ECVAM validation study on in vitro skinirritation tests[J]. Altex, 2004, 21 (3): 107-114.
[7]
Terada D, Yokoyama Y, Hattori S, et al. The outermost surface properties of silk fibroin films reflect ethanol-treatment conditions used in biomaterial preparation[J]. Materials Science & Engineering C-Materials for Biological Applications,2016,58:119-126.
[8]
Kim EY, Tripathy N, Cho SA, et al. Bioengineered neo-corneal endothelium using collagen type-I coated silk fibroin film[J].Colloids and surfaces, 2015,136:394-401.
Miles A, Berthet A, Hopf N B, et al. A new alternative method for testing skin irritation using a human skinmodel: a pilot study[J]. Toxicology in vitro,2014,28(2):240-247.
[11]
Katoh M, Hamajima F, Ogasawara T, et al. Assessment of human epidermal model LabCyte EPI-MODEL for in vitro skin irritation testing according to European Centre for the Validation of Alternative Methods (ECVAM)-validated protocol[J]. The journal of toxicological sciences, 2009,34(3):327-334.
[12]
Hang Y, Zhang Y, Jin Y, et al. Preparation of regenerated silk fibroin/silk sericin fibers by coaxial electrospinning[J]. International journal of biological macromolecules, 2012,51(5):980-986.
[13]
Nogueira G M,Rodas A C, Leite C A, et al. Preparation and characterization of ethanol-treated silk fibroin dense membranes for biomaterials application using waste silk fibers as raw material[J].Bioresource technology,2010,101(21):8446-8451.
Fernando P M, Piao M J, Kang K A, et al. Rosmarinic Acid Attenuates Cell Damage against UVB Radiation-Induced Oxidative Stress via Enhancing Antioxidant Effects in Human HaCaT Cells [J]. Biomol the rapeutics, 2016, 24(1): 75-84.
[16]
Hou W, Gao W, Wang D, et al. The Protecting Effect of Deoxyschisandrin and Schisandrin B on HaCaT Cells against UVB-Induced Damage [J]. Plos one, 2015, 10(5): 127-177.
2016, Vol. 27 
