Effects of ulinastatin on cytokines level in plasma following the both lower extremities ischemic reperfusion
WANG Naitian1, YU Mingjun2, LIU Lijin3, and TU Weifeng4
1.Department of Anesthesiology, General Hospital of Chinese People’s Armed Police Force, Beijing 100039, China;
2.The Medical Team of the Detachment Three of Hebei Province Corps,Chinese People’s Armed Police Force, Qinhuangdao 066100, China;
3.Graduate School,Jinzhou Medical University 121001, China;
4.Department of Anesthestiolgy,Guangzhou General Hospital of Guangzhou Military Command, Guangzhou, 510010, China
Objective To investigate the effects of ulinastatin (UTI) on plasma cytokines following intermittent continuous ischemic reperfusion(IR, cumulative time >150min ) of both lower extremities.Methods Thirty patients, both low extremities bound with tourniquets during operation, were randomly divided into three groups: normal control group(Group NC), UTI pretreatment group 1(Group UP1) and group 2(Group UP2). The patients in Group UP1 were intravenously infused with UTI 6000 U/kg 15 min before the tourniquet application and the last tourniquet loosening, respectively. The patients in Group UP2 were intravenously infused with UTI 12 000 U/kg 15 min only before the last tourniquet loosening, UTI was diluted with 100 ml sodium chloride and infused intravenously within 10 minutes. The patients in Group NC were administered with the same volume sodium chloride. At the time, 20 min before tourniquet apptionhor(before IR); 30 min after last tourniquet lossening(30 min after IR); and 1 d, 3 d, 7 d after IR, the peripheral blood was sampled to measure plasma levels of C reactive protein(CRP), interleukin-6(IL-6), and tumor necrosis factor-α(TNF-α).Results The levels of CRP, IL-6 and TNF-α in the three groups all rose significantly 30 min, 1 d and 3 d after IR compared with those before IR respectively(P<0.05). But the levels of the cytokines in Group UP1 and UP2 were significant lower than those in Group NC[CRP:1 d(11.15±2.32)、(10.48±2.08)mg/l vs NC(15.83±4.56)mg/l,3 d (4.29±1.89)、(3.89±1.34)mg/l vs NC(6.78±2.95)mg/l;TNF-α:1d(31.24±8.66)、(29.72 ±8.77)ng/l vs NC(44.802 ±11.70)ng/l,3 d (13.75±5.11)、(15.73±4.16)ng/l vs NC(20.787±8.05)ng/l;IL-6:1 d(160.80±46.95)、(179.72±35.77)ng/l vs NC(329.50±95.34)ng/l,3 d (45.32 ±16.53)、(53.35±17.62)ng/l vs NC(79.33±24.93)ng/l,P<0.05]. There were no significant differences between Group UP1 and Group NC in each time in terms of the cytokines(P>0.05). The inflammatory cytokines recovered to the same level 7 d after IR.Conclusions UTI pretreatement may relieve the system inflammatory reaction following both low extremities, prevent the increases of plasma cytokines after IR, thus may attenuate organ damage mediated by cytokines.
王乃田, 于明军, 刘理金, 屠伟峰. 乌司他丁预先给药对双下肢缺血再灌注损伤后血浆细胞因子的影响[J]. 武警医学, 2016, 27(8): 810-813.
WANG Naitian, YU Mingjun, LIU Lijin, and TU Weifeng. Effects of ulinastatin on cytokines level in plasma following the both lower extremities ischemic reperfusion. Med. J. Chin. Peop. Armed Poli. Forc., 2016, 27(8): 810-813.
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2016, Vol. 27 
