1.Oncology Department of Suizhou Central Hospital, Affiliated Hospital of Hubei University of Medicine, Suizhou 441300, China; 2.Tumor Radiotherapy Department of Affiliated Hospital of Nantong University, Nantong 22600, China; 3. Clinical Medicine Department of Hubei University of Medicine, Shiyan 442000, China
Abstract：Objective To investigate the effects of down-regulation of forkhead box protein M1 (FoxM1) expressions by shRNA on proliferation and apoptosis of esophageal squamous cell carcinoma (ESCC) cells.Methods Plasmid that highly expressed FoxM1-shRNA was successfully constructed and transfected with TE1 cell line. FoxM1 was analyzed by Western blot. Cell proliferation was tested by cell counting kit-8 while cell cycle and apoptosis were analyzed by flow cytometry and Annexin-V-PE/7-AAD staining kit.Results FoxM1 expressions in ESCC cells transfected with specific shRNA were significantly down-regulated at the protein level and cell proliferation was inhibited in a time-dependent manner. The inhibition rate was 68.0%±6.4% (P<0.05), with cell cycle arrested at G1 phase arrest (59.14%±1.69% vs 40.51%±1.45%,t=14.23,P<0.01)and the apoptosis rate of TE1 cells reached 35.37% (2.48%±0.49% vs 35.37%±0.56%,t=76.56,P<0.01).Conclusions Interventions with FoxM1 gene expressions can inhibit the proliferation and apoptosis of ESCC cells, suggesting that FoxM1 can be a novel therapeutic target for ESCC.
Wu X, Gu X, Han X, et al . A novel function for FoxM1 in interkinetic nuclear migration in the developing telencephalon and anxiety-related behavior [J]. J Neurosci, 2014, 34(4): 1510-1522.
[6]
Huang C, Xie D, Cui J, et al . FOXM1c promotes pancreatic cancer epithelial to mesenchymal transition and metastasis via upregulation of expression of the urokinase plasminogen activator system [J]. Clin Cancer Res, 2014, 20(6): 1477- 1488.
[7]
Xia L, Huang W, Tian D, et al . Overexpression of forkhead box C1 promotes tumor metastasis and indicates poor prognosis in hepatocellular carcinoma [J]. Hepatology, 2013, 57(2): 610-624.
[8]
Kong F F, Qu Z Q, Yuan H H, et al . Overexpression of FOXM1 is associated with EMT and is a predictor of poor prognosis in non-small cell lung cancer [J]. Oncol Rep, 2014, 31(6): 2660-2668.
[9]
Arora R, Yates C, Gary B D, et al . Panepoxydone targets NF-kB and FOXM1 to inhibit proliferation, induce apoptosis and reverse epithelial to mesenchymal transition in breast cancer [J]. PLoS One, 2014, 9(6): e98370.
[10]
Wen N, Wang Y, Wen L, et al . Overexpression of FOXM1 predicts poor prognosis and promotes cancer cell proliferation, migration and invasion in epithelial ovarian cancer [J]. J Transl Med, 2014, 12(134): 1-13.
[11]
Cheng A L, Kang Y K, Chen Z, et al . Efficacy and safety of sorafenib in patients in the Asia-Pacific region with advanced hepatocellular carcinoma: a phase III randomised, double-blind, placebo-controlled trial [J]. Lancet Oncol, 2009, 10(1): 25-34.
[12]
Lee Y L, Ahn B C, Lee Y, et al . Targeting of hepatocellular carcinoma with glypican-3 targeting peptide ligand [J]. J Pept Sci, 2011, 17(11): 763-769.
[13]
Li X R, Chu H J, Lv T, et al . miR-342-3p suppresses proliferation, migration and invasion by targeting FOXM1 in human cervical cancer [J]. FEBS Lett, 2014, 588(17): 3298-3307.
[14]
Zhou J, Wang Y, Wang Y, et al . FOXM1 modulates cisplatin sensitivity by regulating EXO1 in ovarian cancer [J]. PLoS One, 2014, 9(5): e96989.
[15]
Miao L, Xiong X, Lin Y, et al . Down-regulation of FoxM1 leads to the inhibition of the epithelial-mesenchymal transition in gastric cancer cells [J]. Cancer Genet, 2014, 207(3): 75-82.
[16]
Chen T, Xiong J, Yang C, et al . Silencing of FOXM1 transcription factor expression by adenovirus-mediated RNA interference inhibits human hepatocellular carcinoma growth [J]. Cancer Gene Ther, 2014, 21(3): 133-138.
[17]
Wang I C, Meliton L, Ren X, et al . Deletion of Forkhead Box M1 transcription factor from respiratory epithelial cells inhibits pulmonary tumorigenesis [J]. PLoS One, 2009,4(8):e6609.
2017, Vol. 28 
