Effect of dexamethasone on body composition in mice
WANG Yuebing1, LIU Qingchun1, LU Feixiang1, LI Jun2, WANG Ying1
1. Department of Nutrition, General Hospital of Chinese People's Armed Police Force,Beijing 100039,China; 2. Snpply Center,General Hospital of Chinese People's Armed Police Force,Beijing 100039,China; 3. Laboratory Animal Center, Peking University, Beijing 100871, China
Abstract：Objective To explore the effect of dexamethasone on body composition of mice of two different ages in order to select an ideal sarcopenia model. Methods C57BL/6 mice aged 8-10 weeks were randomly divided into the experimental group(DA) and control (NA),while another sixteen C57BL/6 mice aged 6-7 months were randomly assgined to the experimental group(DM) and control (NM). There were eight mice in each group.The control group received 0.9% saline injection, while the experimental group was subcutaneously injected with 5 mg/kg dexamethasone for 19 days. The body composition of all the mice was measured every three days. 24h after the final administration,the body composition was measured with EchoMRITM. Results After dexamethasone intervention, compared with NA group, weight in DA group was reduced by 5.97% (P<0.05), muscle mass by 14.33%(P<0.01)but fat mass increased by 32.94%(P<0.05).Compared with NM group, weight in DM group increased by 13.67% (P<0.05), muscle mass reduced by6.06%(P<0.05)and fat mass increasedby 43.77%(P<0.01). There was no significant difference in the intervention effect of dexamethasone on muscle between mice of 6-7 months old and those of 8-10 weeks old(P>0.05), but the intervention effect of dexamethasone on fat was significantly different (P< 0.05).Fat of mice of 6-7 months old was increased more significantly than that of 8-10-week-old mice. Conclusions Under the conditions of this experiment,mice of 6-7 months old are more suitable for a long-term study of a sarcopenia model than mice of 8-10 weeks old.
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WANG Yuebing, LIU Qingchun, LU Feixiang, LI Jun, WANG Ying. Effect of dexamethasone on body composition in mice. Med. J. Chin. Peop. Armed Poli. Forc., 2017, 28(11): 1093-1095.
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