Effects of Yi Qi Yang Yin Huo Xue decoction on miR-375 in pancreatic tissue and on functions of β cells in diabetic rat models
YUAN Jie1,ZHANG Jun2,DAI Jinying1,WANG Shunyi1, XU Xuemei1,ZHAO Jing1,and LIU Ying2
1.North China University of Science and Technology, Tangshan, 063000, China ; 2.Department of Endocrinology, Tangshan Hospital of Traditional Chinese Medicine,Tangshan 063000, China
Abstract：Objective To study the effect of Yi Qi Yang Yin Huo Xue decoction on miR-375 in pancreatic tissue and on the function of β cells in diabetic rat models.Methods Diabetic rat models were established using high-energy diet and intravenous injection of streptozocin(STZ).The rats were divided into blank group,model group,and high-,medium- and low-dose Yi Qi Yang Yin Huo Xue decoction groups. After 8 weeks of administration,body weight ( BW) was measured and blood was collected for detection of fasting blood glucose (FBG),fasting blood insulin(FINS),insulin sensitive index(ISI) and for calculation of the indexes of insulin resistance(HOMA-IR). Before the rats were sacrificed,2-hour plasma glucose(2hPG) was determined. The histopathological features in the islet tissue were examined with HE staining. The level of miR-375 in the islet tissue was detected with qPCR. Single factor analysis of variance was used to sort out the data.Results After the treatment with Yi Qi Yang Yin Huo Xue decoction, the levels of WB,FBG,2hPG,FINS and HOMA-IR decreased significantly (P<0.05) compared with the model group while the ISI was increased significantly(P<0.05).The islet apoptosis was reduced by the administration of this decoction.Compared with model group (2.29±0.91),the levels of miR-375 in the high dose Yi Qi Yang Yin Huo Xue decoction group(0.48±0.19),medium dose group(0.43±0.32)and low dose group(0.33±0.21)were decreased significantly(P<0.05).Conclusions Yi Qi Yang Yin Huo Xue decoction can improve islet beta cell function,and the mechanism may be associated with the down-regulation of the level of miR-375 in islet tissues.
袁 洁,张 军,戴金颖,王顺意,徐雪梅,赵 静,刘 颖. 益气养阴活血方对2型糖尿病大鼠胰腺miR-375表达水平和胰岛功能的影响[J]. 武警医学, 2017, 28(4): 347-351.
YUAN Jie,ZHANG Jun,DAI Jinying,WANG Shunyi, XU Xuemei,ZHAO Jing,and LIU Ying. Effects of Yi Qi Yang Yin Huo Xue decoction on miR-375 in pancreatic tissue and on functions of β cells in diabetic rat models. Med. J. Chin. Peop. Armed Poli. Forc., 2017, 28(4): 347-351.
Yang W,Lu J,Weng J,et al.Prevalence of diabetes among men and women in China[J].N Engl J Med,2010,362(12):1090-1101.
[5]
Lahmy R,Soleimani M,Sanati M H,et al. miRNA-375 promotes beta pan-creatic differentiation in human induced pluripotent stem ( hiPS ) cells[J].Molecular Biol Rep,2014,41(4):2055-2066.
[6]
Nathan G,Kredo-Russo S,Geiger T,et al.MiR-375 promotes redifferentiation of adult human β cells expanded in vitro[J].PLoS One,2015,10(4):e0122108.
[7]
Sun K,Chang X,Yin L,et al.Expression and DNA methylation status of microRNA-375 in patients with type 2 diabetes mellitus[J].Mol Med Rep,2014, 9(3):967-972.
[8]
Lorenzo C,Wagennecht L E,D’Agostino R B Jr,et al.Insulin Resistence,bete-cell dysfunction and conversion to type 2 diabetes in a multiethnic population: the Insulin Resistence Atherosclerosis Study[J].Diabetes Care,2010,33(1):67-72.
[9]
Porte D Jr,Kahn S E.β-Cell dysfunction and failure in type 2 diabetes: potential mechanisms[J].Diabetes, 2001,50( Suppl 1):S160-S163.
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