Abstract：Objective To analyze the mechanism by which TGF beta/STAT signaling pathway is involved in simulating the proliferation of periodontal ligament stem cells stimulated by orthodontic static pressure.Methods The periodontal ligament stem cells were cultured in vitro to grow well before being pressed (100 KPa, 1 H, 6 h and 12 h) for different lengths of time. Western blotting was used to detect the expressions of apoptosis related proteins and TGF/STAT signaling pathway proteins.Results In vitro cultured human periodontal ligament stem cells had multiple differentiation abilities. After 1 h and 6 h of treatment with pressure, the Bax increased significantly (0.34±0.05 vs 0.42±0.08, P<0.05), while the level of Bcl2 decreased significantly (0.33±0.05 vs 0.29±0.05, P<0.05). The expressions of TGF beta/STAT signaling protein TGF beta (0.34±0.06 vs 0.39±0.07, P<0.05)and STAT2 increased significantly(0.32±0.06 vs 0.43±0.08, P<0.05). There was no significant difference in the expressions of these proteins in stem cells treated for 12 hours (P>0.05).Conclusion Simulated orthodontic static pressure stimulation can significantly inhibit the proliferation of periodontal ligament stem cells in vitro, which may be related to abnormality of TGF beta/STAT signaling pathway.
2019, Vol. 30 
