Correlations between hAOX expressions and clinical features of patients with renal carcinoma
GAO Wanfeng1, YU Xinlu1, LI Qing2, ZHANG Shanshan1, SONG Bo3, WANG Yue4, SU Hong1
1.Department of Urology,2.Medical Affairs Office,3.Department of Pharmacy,4.Department of Rehabilitation,Liaoning Provincial Corps Hospital,Chinese People’s Armed Police Force, Shenyang 110034, China
Abstract：Objective To investigate the expression of the aldehyde oxidase (human aldehyde oxidase, hAOX) in renal cell carcinoma and its correlations with clinical biological behavior.Methods Immunohistochemistry was used to detect the expression of hAOX in 86 cases of renal carcinoma and normal renal histopathological specimens, including 64 cases of clear cell carcinoma, 18 cases of papillary renal cell carcinoma and 4 cases of granulosa cell carcinoma. TNM staging criteria defined 26 cases in stage Ⅰ, 31 cases in stage Ⅱ, and 29 cases in stage Ⅲ+Ⅳ. There were 31 cases with lymph node metastasis and 55 cases without lymph node metastasis. Prism 6.0 statistical software was used to analyze the relationships between hAOX and clinicopathological factors and 5-year survival rate.Results The positive expression rate of immunohistochemical hAox was 22.09% in 86 cases of renal carcinoma, compared to 85.0% in normal tissues, which was significantly higher than that in renal carcinoma tissues. The expression of hAox was independent of the age, gender, tissue type and tumor diameter of patients with renal cancer. However, it was significantly correlated with lymph node metastasis, TNM stage and ki-67 expression. The 5-year survival rate of patients with hAox (+) expression was significantly higher than that of patients with hAox (-) expression. Moreover, the prognosis of patients with hAox (+) expression was significantly better than that of patients with hAox (-) expression.Conclusion The expression of hAOX is correlated with the occurrence, development and prognosis of renal cancer, plays an important role in maintaining the normal growth of cells, and facilitates clinical diagnosis, treatment and prognosis.
高万峰, 于新路, 李青, 张珊珊, 宋博, 王雪, 苏宏. hAOX 在人肾癌组织中的表达及其与临床生物学行为的关系[J]. 武警医学, 2019, 30(5): 406-408.
GAO Wanfeng, YU Xinlu, LI Qing, ZHANG Shanshan, SONG Bo, WANG Yue, SU Hong. Correlations between hAOX expressions and clinical features of patients with renal carcinoma. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(5): 406-408.
Garattini E, Fratelli M, Terao M. Mammalian aldehyde oxidases:genetics,evolution and biochemistry[J]. Hepatology, 2008(7/8):1602-1609.
[2]
Morjwaki Y,Yamam T,Takahashi S, et al.Widespread cellular distribution of aldehyde oxidase in human tissues found by immunohistoehemistfy staining[J].Histol Histopathol, 2001,16:745-753.
[3]
Neumeier M,Weigert J,Schaffler A. Aldehyde oxidase 1 is highly abundant in hepatic steatosis and is downregulated by adiponectin and fenofibric acid in hepatocytes in vitro[J].Biochem Biophys Res Commun,2016,350(3):73l-735.
[4]
Coleman M. Vontas J G, Hemingway J.Molecular characterization of the amplified aldehyde oxidase fromin- secticide resistant Culex quinquefasciatus[J]. Cell Proteomics, 2002(3):2149-2153.
[5]
Garattini E, Fratelli M, Terao M. Mammalian aldehyde oxidases : genetics, evolution and biochemistry [J]. Hepatology, 2008(7/8): 1602-1609.
[6]
回允中(译). 阿克曼外科病理学[M]. 北京:人民卫生出版社,2009:1603-1615.
[7]
Singletary S E,Allred C,Ashley P,et al. Staging system for renal cancer:revisions for the 6th edition of the AJCC Cancer Staging Manual[J]. Surg Clin North Am,2003,83(4):803-810.
[8]
Li S Y, Hu J A, Wang H M,et al. Expression of Yes-associated protein I gene and protein in oral spuamous cell carcinoma [J].Chin Med J(Engl), 2013,126(4):655-658.
[9]
Schumann S, Terao M, Garattini E. Site directed mutagenesis of amino acid residues at the active site of mouse aldehyde oxidase AOX[J]. Int J Clin Pract, 2009,4:2056-2062.
[10]
Delpuech O,Trabut J B, Carnot F, et al. Identification,using cDNA macroarray analysis,of distintc gene expression profiles associated with pathological and virological features of hepatocelluar carcinoma[J]. Oncogene,2002,21(18):2926-2937.
[11]
Neumeier M,Weigert J,Schaffler A. Aldehyde oxidase 1 is highly abundant in hepatic steatosis and is downregulated by adiponectin and fenofibric acid in hepatocytes in vitro[J].Biochem Biophys Res Commun,2016, 350(3):731-735.
[12]
Shaw S,Jayatilleke E.The role of aldehyde oxidase in ethanolinduced hepatic lipid pemxidation in the rat[J].Biochem J,1990,268:579-583.
Huang D,Yichikawa Y.Two different enzymes are primarily responsible for retinoie acid is in rabbit liver cytosol [J]. Bio-chem Biophys Res Commun,1994,205:1278-1283.
[15]
Hall W W, Krenitsky T. A Aldehyde oxidaee from rabbit liver:specificity toward purines and theiranalogs[J].J Hepathol, 2010(1):225-231.
2019, Vol. 30 
