Effects of clemastine fumarate on hypoxic lung injury in rats at high altitude
ZHOU Kun1,2,3, GAO Wenjie1, LI Hong3
1. Logistics University of People’s Armed Police Force,Tianjin 300309,China; 2. Chengdu First Center for Retired Officers of Sichuan Military Command,Chengdu 610041,China; 3. Department of Anesthesiology, Characteristic Medical Center of the Chinese People’s Armed Police Force,Tianjin 300162,China
Abstract：Objective To explore the effect of clemastine fumarate on hypoxic lung injury in rats at high altitude.Methods Forty eight-week-old male SD rats were equally divided into the control group (group C), high altitude hypoxia lung injury group (HH group), dexamethasone treatment group (DXM group) and clemastine fumarate treatment group (CLE group) using the random number table method. The HH group, DXM group and CLE group were put into an animal experiment cabin separately in a low-pressure and hypoxic mode. The DXM group was intramuscularly injected with 2 mg/kg DXM every 6 hours starting from one hour before entry. The CLE group was intramuscularly injected with 0.9 mg/kg 2 h before entry, and another dose of 0.9mg/kg was given 10 hours after entry into the cabin, thus completing the establishment of the model. The rats in each group were immediately sacrificed before the wet/dry weight ratio (W/D) of the upper lobes of right lungs was measured. HE staining was performed on the tissues of the lower lobes of right lungs. The mRNA levels of interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), toll-like receptor 4 (TLR4) and nuclear factor κB (NF-κB) in lung tissues were detected by PCR. Expression levels of IL-1β, TNF-α, TLR4 and NF-κB were determined by Western blot.Results Compared with the control group, the W/D ratios of the HH group, DXM group and CLE group were significantly increased (P<0.05), so were the mRNA transcription and protein expression levels of IL-1β, TNF-α, TLR4 and NF-κB in lung tissues (all P<0.05). Compared with the HH group, the W/D ratios of DXM group and CLE group were significantly decreased (P<0.05), so were mRNA transcription and protein expression levels of IL-1β, TNF-α, TLR4 and NF-κB (all P<0.05).Conclusions Clemastine fumarate can reduce the degree of high altitude hypoxic lung injury in rats.
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2020, Vol. 31 
