Abstract：Objective To investigate the expression of placenta growth factor (PlGF) in lung tissue of chronic asthma rats.Methods Forty-five male Wistar rats were randomly divided into three groups. Rats in group A were given no special treatment.Rats in group B were sensitized with OVA and challenged with saline.Rats in group C were sensitized and challenged with OVA to establish a chronic asthma model. The expression of PlGF in BALF was detected using ELISA.Lung sections were stained with hematoxylin and eosin (HE), periodic acid Schiff (PAS), and Masson trichrome, and the images were analyzed morphometrically. The expression of PlGF in the airways was detected withimmunohistochemistry.Results The expression of PlGF in the airways of rats in group C was up-regulated more significantly than in group A and group B(*P<0.01, **P<0.01),and more significantly in BALF of chronic asthma rats (group C) than in group A and group B(P<0.05).Conclusions The expression of PlGF is upregulated in the lung tissue of chronic asthma rats.
Bobic S, Seys S, De Vooght V, et al. Placental growth factor contributes to bronchial neutrophilic inflammation and edema in allergic asthma[J]. Am J Respir Cell Mol Biol,2012,46(6): 781-789.
[2]
Cheng S L, Wang H C, Yu C J, et al. Increased expression of placenta growth factor in COPD[J]. Thorax,2008,63(6): 500-506.
[3]
Palmans E, Kips J C, Pauwels R A. Prolonged allergen exposure induces structural airway changes in sensitized rats[J]. Am J Respir Crit Care Med,2000,161(2 Pt 1): 627-635.
[4]
Sumi Y, Foley S, Daigle S, et al. Structural changes and airway remodelling in occupational asthma at a mean interval of 14 years after cessation of exposure[J]. Clin Exp Allergy,2007,37(12): 1781-1787.
[5]
Snibson K J, Bischof R J, Slocombe R F,et al. Airway remodelling and inflammation in sheep lungs after chronic airway challenge with house dust mite[J]. Clin Exp Allergy,2005,35(2): 146-152.
[6]
Leigh R, Ellis R, Wattie J, et al. Dysfunction and remodeling of the mouse airway persist after resolution of acute allergen-induced airway inflammation[J]. Am J Respir Cell Mol Biol,2002,27(5): 526-535.
Wu D, Lai T, Yuan Y, et al. Elevated expression of placental growth factor is associated with airway-wall vascular remodelling and thickening in smokers with asthma[J]. Sci Rep,2017,7:43017.
[9]
McDonald D M. Angiogenesis and remodeling of airway vasculature in chronic inflammation[J]. Am J Respir Crit Care Med,2001,164(10 Pt 2): S39-45.
[10]
Yancopoulos G D, Davis S, Gale N W, et al. Vascular-specific growth factors and blood vessel formation[J]. Nature,2000,407(6801): 242-248.
[11]
Tsao P N, Li H, Wei S C, et al. Expression of angiogenic factors and their receptors in postnatal mouse developing lung[J]. J Formos Med Assoc,2004,103(2):137-143.
[12]
Clarke A H,Thomas W R,Rolland J M,et al.Murine allergic respiratory responses to the major house dust mite allergen Der p 1[J]. Int Arch Allergy Immunol,1999,120(2):126-134.
[13]
Zhang L, Zhao S, Yuan L, et al. Knockdown of placental growth factor (PLGF) mitigates hyperoxia-induced acute lung injury in neonatal rats: Suppressive effects on NFκB signaling pathway[J]. Int Immunopharmacol,2016,38: 167-174.
[14]
Wong C W, Cheung N, Ho C, et al. Characterisation of the inflammatory cytokine and growth factor profile in a rabbit model of proliferative vitreoretinopathy[J]. Sci Rep,2019,9(1): 15419.
[15]
Noma H, Mimura T, Yasuda K, et al. Role of soluble vascular endothelial growth factor receptors-1 and -2, their ligands, and other factors in branch retinal vein occlusion with macular edema[J]. Invest Ophthalmol Vis Sci,2014,55(6): 3878-3885.
[16]
Liu C Y, Qin S, Liu L Y, et al. Inhibitory Effect of Acupoint Application on Airway Remodeling and Expression of TGF-β 1/Smad 3 in the Lung Tissue of Chronic Asthma Mice[J]. Zhen Ci Yan Jiu,2017,42(2): 153-158.
[17]
Stumm C L, Halcsik E, Landgraf R G,et al. Lung remodeling in a mouse model of asthma involves a balance between TGF-β1 and BMP-7[J]. PLoS One,2014,9(4): e95959.
2020, Vol. 31 
