Effects of miR-30a-5p on proliferation, migration and invasion of non-small cell lung cancer cells by directly targeting NUAK1
LI Aiming1, LIU Ke2, WANG Yuanyuan2, WANG Qian3
1. Cardiothoracic Vascular Surgery, 3. Department of Pediatrics,Baoding First Hospital, Baoding 071000,China, 2. Department of Ultrasound,Baoding First Central Hospital, Baoding 071000,China
Abstract：Objective To investigate the expressions of miR-30a-5p and its target gene NUAK1 in non-small cell lung cancer, and the effects on the proliferation, migration and invasion of A549 cells.Methods The specimens of 64 cases of NSCLC were collected.QPCR and Western blot were used to detect the expressions of miR-30a-5p and NUAK1 in NSCLC tissues and cell lines. The relationship between miR-30a-5p and NUAK1 was predicted via TargetScan website and was verified by dual luciferase reporter assay and Western blot. The effects of miR-30a-5p on proliferation, migration and invasion of A549 were determined by MTT and Transwell assays.Results The expression of miR-30a-5p in NSCLC tissues (0.33±0.14) was significantly lower than in adjacent tissues (1.00±0.21) (t=21.237, P<0.05), while the expressions of NUAK1 mRNA (4.13±1.87) and protein (3.41±1.62) in NSCLC tissues were significantly higher than in adjacent tissues (1.00±0.17, 1.00±0.16) (t=13.335, 11.844, P<0.05). The expression of miR-30a-5p in NSCLC cells H460, H1299 and A549 (0.35±0.03, 0.51±0.05, 0.28±0.03) was significantly lower than in normal human lung epithelial cells BEAS-2B (1.00±0.08) (F=77.100, P<0.05). The expressions of NUAK1 mRNA (2.98±0.30, 2.39±0.24, 3.42±0.35) and protein (3.06±0.31, 2.27±0.23, 4.12±0.41) in H460, H1299 and A549 cells were significantly higher than in BEAS-2B cells(1.00±0.09, 1.00±0.11) (F=46.660, 63.070, P<0.05).Conclusions miR-30a-5p can inhibit the proliferation, migration and invasion of A549 cells by targeting NUAK1, suggesting that miR-30a-5p and NUAK1 may become new targets for diagnosis and treatment of NSCLC.
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LI Aiming, LIU Ke, WANG Yuanyuan, WANG Qian. Effects of miR-30a-5p on proliferation, migration and invasion of non-small cell lung cancer cells by directly targeting NUAK1. Med. J. Chin. Peop. Armed Poli. Forc., 2020, 31(7): 595-600.
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2020, Vol. 31 
