Abstract：Objective To investigate the effect of curcumin on oxidative stress in lung induced by thoracic blast exposure and its possible molecular mechanism.Methods Thirty C57BL/6 mice were randomly divided into control group,model group and model with curcumin group.Collect lung tissue at 24 h after thoracic blast exposure.The left lung was fixed in 10% formalin and embedded in paraffin.The right lung was preserved at -80 ℃.Hematoxylin-eosin staining was used to detect the pathological changes of lung tissue,and ROS was used to detect the oxidative stress injury.Western blot was used to detect the expression of MDA5,SOD-1,IRE-α,p-PI3K,Nrf2 and Keap1.Results After chest blast,the cell arrangement of lung tissue in model group was obviously disordered,cell degeneration and inflammatory cell infiltration were observed.Compared with the control group,the blue fluorescence decreased and the red fluorescence increased significantly in the model group under UV excitation.Western blot showed that the relative density of MDA5,IRE-α,SOD-1,Nrf2 and Keap1 in the model group were 0.68±0.08,0.63±0.04,0.38±0.02,0.32±0.03 and 0.98±0.11,respectively.These proteins in the control group were 0.33±0.03,0.29±0.02,0.81±0.09,0.43±0.05 and 0.44±0.04,respectively.The relative density of p- PI3K/PI3K in the model group was 0.74±0.05,which was significantly higher than that of the control group (0.30±0.02).The differences above were statistically significant (P<0.05).After curcumin intervention,comparing with the model group,HE staining showed that the degeneration and necrosis of lung tissue and the infiltration of inflammatory cells were alleviated.ROS detection showed that the blue fluorescence increased and the red fluorescence decreased significantly in the model with curcumin group.Western blot showed that the relative density of MDA5,IRE-α,SOD-1,Nrf2 and Keap1 in model with curcumin group were 0.48±0.07,0.36±0.06,0.51±0.07,0.71±0.08 and 0.53±0.06,respectively,and the relative density of p-PI3K/PI3K was 0.56±0.07.The above differences were statistically significant compared with the model group (P<0.05).Conclusions Curcumin can protect lung tissue from oxidative stress injury induced by thoracic blast exposure,and the mechanism may be associated with PI3K/Nrf2/Keap1 signaling pathway.
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