Expression and clinical significance of ABCA1 in ovarian cancer
XIE Chengmao, ZHOU Qi
Department of Gynecology, Beijing Obstetrics and Gynecology Hospital affiliated to Capital Medical University. Beijing Maternal and Child Health Care Hospital, Beijing 100026, China
Abstract：Objective To investigate the expression and clinical significance of ATP binding cassette transporter A1 (ABCA1) in ovarian cancer.Methods Two hundred and twelve patients who were admitted into Beijing Obstetrics and Gynecology Hospital from January 2016 to December 2020 were selected , (106 patients with ovarian cancer as the experimental group and 106 patients with benign ovarian tumor as the control group). Fasting elbow venous blood was collected routinely, and the blood lipid contents (triglyceride (CHO), total cholesterol (TG), high density lipoprotein (HDL) and low-density lipoprotein (LDL)between the two groups were analyzed by enzyme oxidation method; tissue samples were collected immediately after surgical resection. The expression of ABCA1 in tissues was detected by immunohistochemistry and western-blot; Kaplan Meier survival analysis was used to estimate the relationship between ABCA1 expression and patient survival, and log rank method was used for further verification.Results Compared with the control group, the blood lipid levels (CHO, TG, HDL, LDL) in peripheral blood of patients with ovarian cancer were significantly lower (P<0.01); ABCA1 was expressed in ovarian cancer and benign ovarian tumors, and the expression in ovarian cancer (0.15±0.02) was significantly higher than that in the control group (0.06±0.01, P<0.01).Conclusions ABCA1 is closely related with the pathogenesis of ovarian cancer, which may participate in the occurrence and development of ovarian cancer by regulating the lipid metabolism of ovarian cancer, and then affect the prognosis of patients.
Salehi F, Dunfield L, Phillips K P, et al. Risk factors for ovarian cancer: an overview with emphasis on hormonal factors[J]. J Toxicol Environ Health B Crit Rev, 2008, 11(3-4):301-321.
Currie E, Schulze A, Zechner R, et al. Cellular fatty acid metabolism and cancer[J]. Cell Metab, 2013,18(2):153-161.
[4]
Zhang F, Du G. Dysregulated lipid metabolism in cancer[J]. World J Biol Chem, 2012,3(8):167-174.
[5]
Salamon R S, Backer J M. Phosphatidylinositol-3,4,5-trisphosphate: tool of choice for class I PI 3-kinases[J]. Bioessays, 2013,35(7):602-611.
[6]
Cantor J R, Sabatini D M. Cancer cell metabolism: one hallmark, many faces[J]. Cancer Discov, 2012,2(10):881-898.
[7]
Park J B, Lee C S, Jang J H, et al. Phospholipase signalling networks in cancer[J]. Nat Rev Cancer, 2012,12(11):782-792.
[8]
Nomura D K, Long J Z, Niessen S, et al. Monoacylglycerol lipase regulates a fatty acid network that promotes cancer pathogenesis[J]. Cell, 2010,140(1):49-61.
[9]
Nieman K M, Romero I L, Houten B, et al. Adipose tissue and adipocytes support tumorigenesis and metastasis[J]. Biochim Biophys Acta, 2013,1831(10):1533-1541.
[10]
Blackstone E, Morrison M, Roth M B. H2S induces a suspended animation-like state in mice[J]. Science, 2005,308(5721):518.
[11]
Bhattacharyya S, Saha S, Giri K, et al. Cystathionine beta-synthase (CBS) contributes to advanced ovarian cancer progression and drug resistance[J]. PLoS One, 2013,8(11):e79167.
[12]
Namekata K, Enokido Y, Ishii I, et al. Abnormal lipid metabolism in cystathionine beta-synthase-deficient mice, an animal model for hyperhomocysteinemia[J]. J Biol Chem, 2004,279(51):52961-52969.
[13]
Tania M, Khan M A, Song Y. Association of lipid metabolism with ovarian cancer[J]. Curr Oncol, 2010,17(5):6-11.
[14]
Shimano H. Sterol regulatory element-binding proteins (SREBPs): transcriptional regulators of lipid synthetic genes[J]. Prog Lipid Res, 2001,40(6):439-452.
[15]
Ye J, Debose-Boyd R A. Regulation of cholesterol and fatty acid synthesis[J]. Csh Perspect Biol, 2011,3(7):322-330.
[16]
Baenke F, Peck B, Miess H, et al. Hooked on fat: the role of lipid synthesis in cancer metabolism and tumour development[J]. Dis Model Mech, 2013,6(6):1353-1363.
[17]
Greenberg A S, Coleman R A, Kraemer F B, et al. The role of lipid droplets in metabolic disease in rodents and humans[J]. J Clin Invest, 2011,121(6):2102-10.
[18]
Smicun Y, Gil O, Devine K, et al. S1P and LPA have an attachment-dependent regulatory effect on invasion of epithelial ovarian cancer cells[J]. Gynecol Oncol, 2007,107(2):298-309.
[19]
Luciani M F, Chimini G. The ATP binding cassette transporter ABC1, is required for the engulfment of corpses generated by apoptotic cell death[J]. Embo J, 1996,15(2):226-235.
[20]
Kim J S, Romero R, Kim M R, et al. Involvement of Hofbauer cells and maternal T cells in villitis of unknown aetiology[J]. Histopathology, 2008,52(4):457-464.
[21]
Pyragius C E, Fuller M, Ricciardelli C, et al. Aberrant lipid metabolism: an emerging diagnostic and therapeutic target in ovarian cancer[J]. Int J Mol Sci, 2013,14(4):7742-7756.
[22]
Lima R A, Cândido E B, Melo F P, et al. Gene expression profile of ABC transporters and cytotoxic effect of ibuprofen and acetaminophen in an epithelial ovarian cancer cell line in vitro[J]. Rev Bras Ginecol Obstet, 2015,37(6):283-290.
2022, Vol. 33 
