Influencing factors of carrageenan to establish rat models of chronic nonbacterial prostatitis
SU Xuzhen1,2,3, ZHANG Qiang1,2,3, XIANG Ruijun1,2, ZHANG Chunlei1, ZHANG Bin1, and CHANG Dehui1
1. Department of Urology, the 940th Hospital of PLA Joint Logistics Support Force, Lanzhou 730030, China; 2. The First Clinical Medical College of Gansu University of Traditional Chinese Medicine, Lanzhou 730050, China; 3. Key Laboratory of Stem cells and Genetic Drugs of Gansu Province, Lanzhou 730030, China
Abstract：Objective To explore the influencing factors of carrageenan onchronic nonbacterial prostatitis (CNP) modelsin rats. Methods Adult male rats were injected with carrageenan to make models, and the effects of mouse species (SD rats and Wistar rats), injection sites (seminal vesicle andventral lobe of prostate) and injection concentration (1% and 3%) on the modeling were observed.The modeling effect was determined according to the results of HE stainingof prostate histopathological indicators. Results Compared with Wistar male rats, the results of HE staining in SD male prostate tissues were significantly higher (P<0.05). Spontaneous inflammation appeared in parts of normal Wistar male rats (P<0.05), which mightaffect the observation of later experimental results. The effect of bilateral seminal vesicle injection was better than that of left and right ventral prostatic injection(P<0.05), but it increased the mortalityof rats and acute inflammatory reaction occurred; compared with left and right ventral prostaticinjection, the successful rate of model construction was higher, and the local multi-point injection method could make up for the lackof small infiltration area.The modeling effect of 3% carrageenan saline solution was more significant than that of 1% carrageenan saline solution (P<0.05). Conclusions SD male rats are more sensitive to carrageenan than Wistar male rats. A stable and ideal CNP model will be successfully established after 21 days of local injection of 3% carrageenan saline solution into the left and right ventral lobe of the prostate of SD male rats.
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2023, Vol. 34 
