Abstract：Objective To explore the effect of implantation of PLLA nanofibers and BMSCs on spinal cord injury of rats.Methods In vitro, PLLA nanofibers were fabricated and BMSCs were isolated before BMSCs were implanted on the PLLA nanofibers.The Cell Counting Kit-8 was applied to detect the proliferation of BMSCs.The neural differentiation of BMSCs on the PLLA nanofibers induced by RA(retinoic acid) was observed using immunohistochemistry and light microscopy.After the spinal cord injury(SCI) model was prepared, forty-eight rats were randomly and equally assigned to four groups(n=12): the PLLA nanofibers+BMSCs group, PLLA nanofibers group, BMSCs group, and the blank control group.The motor functional recovery of rats was evaluated with the BBB score at 2 w, 4 w, 8 w after operation.Results There was no significant change in the proliferation of BMSCs on PLLA nanofibers compared with culture dishes at 1-day and 4-day.The proliferation of BMSCs on PLLA nanofibers was better than in culture dishes at 7-day.The neural differentiation of BMSCs on PLLA nanofibers was better than in culture dishes.In vivo, there was no significant difference in the rats’ functional recovery after SCI between the PLLA nanofibers+BMSCs group, the PLLA nanofibers group and the BMSCs group at 4-week after operation.The rats’ functional recovery of the PLLA nanofibers+BMSCs group was better than that of the PLLA nanofibers group and the BMSCs group at 8-week after operation.The rats’ functional recovery of the above three groups was better than that of the blank control group at 4-week and 8-week after operation.Conclusions The implantation of PLLA nanofibers and BMSCs can promote the recovery of spinal cord injury of rats.
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