Effect of PLGA scaffold incorporating VEGF and bFGF loaded nanoparticles on wound healing in case of radiation injury
WANG Mu1, ZENG Yong2, ZHANG Lei2, YANG Yan3, GAO Mingmin1
1.Department of Plastic Surgery, 2. Department of Burn Surgery, 3. Department of General Surgery, Shanghai Municipal Corps Hospital, Chinese People’s Armed Police Force, Shanghai 201103,China
Abstract：Objective To study the effects of poly lactic-co-glycolic acid(PLGA) scaffold incorporating VEGF and bFGF loaded nanoparticle complexes on the healing of murine radioactive wounds.Methods Mice undergoing combined application of PLGA and VEGF/bFGF loaded nanoparticle complexes against radioactive wounds were assigned to the experimental group, while those among whom PLGA alone was used to treat radioactive wounds were considered the control group. The wound healing rate and pathological changes of the wounds were observed, while the number of new vascular cells, the area of capillary interception and the number of fibroblasts were detected at different time points after injury between the two groups.Results With regard to the wound surface of mice with radiation injury, the wound healing rate of the experimental group was significantly higher than that of the control group after the combined application of bFGF and VEGF. The expressions of VEGF mRNA and bFGF mRNA in wound tissue of the experimental group were higher than those of the control group, and there was significant difference between the two groups (P<0.05). HE staining performed on the wound tissue suggested that the granulation tissue in the experimental group increased and thickened, and the number and density of fibroblasts were better than those of the control group. Eight and fifteen days after injury, the number and the cross-sectional area of newborn blood capillaries were both increased in the experimental group compared with the control group, so was the number of fibroblasts.Conclusions PLGA nanoparticle complexes loaded with VEGF and bFGF can promote the healing of radioactive wounds in mice.
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2018, Vol. 29 
