Relationships between construction of active immunization against hepatitis B and the changes of Th17 and Treg cells in liver transplant recipients
DOU Gufeng1, ZHU Xiongwei2, CHEN Xinguo2, LU Shuchun3,LI Wei2, HOU Na1, XIAO Hua2, YOU Bo2, SONG Jun1,and NIU Yujian1,2
1.Xuzhou Medical University,Xuzhou 221004, China;
2.The Third Medical Center of PLA General Hospital,Beijing 100039, China;
3.The First Medical Center of PLA General Hospital,Beijing 100853, China
Objective To explore the correlations between the anti-hepatitis B active immune construction and the percentage of peripheral blood Th17 cells and Treg cells in liver transplantation recipients with hepatitis B-related liver disease, and provide theoretical support and practical experience for improving the success rate of construction.Methods One hundred and forty-four liver transplant recipients who were followed up in our hospital from February 2015 to June 2017 were enrolled according to the established criteria. These patients were randomly divided into four groups with informed consent(n=36): traditional group, traditional booster group, fast group and thymosin group. Each group was given a different anti-hepatitis B active immunization induction program. The difference between these groups in the anti-hepatitis B active immunization success rate was compared. The changes of the percentage of Th17 cells and Treg cells in lymphocytes and those of serum hepatitis B surface antibody (anti-HBs) titer were analyzed. The safety of vaccination was observed.Results The success rates of active immunization against hepatitis B in the four groups were 2.78% (1/36), 5.56% (2/36), 22.9% (8/35) and 34.3%(12/35)respectively, so the difference between these groups was statistically significant (P<0.05). The difference between the four groups in terms of increase of anti-HBs titers was significantly higher after the test (P<0.05). The percentage of Th17 cells decreased significantly in each group after the test. There was significant difference between the four groups (P<0.05). There was no significant difference in the percentage of Treg cells between these groups after the test (P>0.05). There was a significant positive correlation between the increase of -HBs and decline of Th17 (r=0.276, P=0.002) but no correlation with Treg changes was observed. Such biochemical parameters as blood routine, urine routine and liver function were not significantly different after the study.Conclusion For liver transplant recipients, it is safe and feasible to construct anti-hepatitis B active immunity via proper inoculation with hepatitis B vaccines. The lower expression of Th17 cells may promote the immune construction against hepatitis B and Treg cells seem to have no effect on the construction.
窦古枫,朱雄伟,陈新国,卢实春,李威,侯娜,肖桦,游波,宋军,牛玉坚. 肝移植受者抗乙肝主动免疫构建与Th17及Treg细胞占比变化的相关性[J]. 武警医学, 2019, 30(2): 105-108.
DOU Gufeng, ZHU Xiongwei, CHEN Xinguo, LU Shuchun,LI Wei, HOU Na, XIAO Hua, YOU Bo, SONG Jun,and NIU Yujian. Relationships between construction of active immunization against hepatitis B and the changes of Th17 and Treg cells in liver transplant recipients. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(2): 105-108.
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2019, Vol. 30 
