Two-dimensional gel electrophoresis analysis of human serum proteome after liver transplantation and FK506 combination therapy
LI Yan1,WANG Hongli2,ZHAO Yongqiang2,Liu Yu3,LIANG Yan1
1. Department of Pharmacy,General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China; 2. National Center of Biomedical Analysis,Beijing 100850, China; 3. Institute of Liver Transplantation, General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China
Abstract：Objective To analyze the serum proteome change after liver transplantation and FK506 combination in human serum by two-dimensional gel electrophoresis (2-DE) and to study the mechanism of immune rejection and the mechanism of action of FK506 combination. Methods The serum samples of 5 liver transplantation patients pre- and post transplantation at 24 hours,10 days,30 days,90 days 180 days were treated using Aurum Serum Protein Mini Kit, and than analyzed by 2-DE. The electrophoresis images were analyzed by using ImageMaster 2D platirum imaging analysis software. Results The differential maps of 2-DE of serum proteome before and after liver transplantation and FK506 combination were established. Protein spots between (877?59.65)-(897.6?61.01),the matching rate before transplantation and different groups after transplantation>70%. Among them 7 protein spots were selected with good repeatability and significant differential expression. Conclusions The 2-DE analytical method of serum proteome after liver transplantation and FK506 combination was preliminarily established. The differential expression protein spots found in the study have laid a foundation for the further study .
2014, Vol. 25 
