Abstract Objective To determine the content of morphine in Yinghuang Powder by HPLC. Methods The analysis was performed on a Hypersil Kromasil C 18 (150 mm×4.6 mm, 5 μm) column at room temperature with a mobile phase consisting of methyl cyanide -0.01 mol/L dibasic potassium phosphate -0.005 mol/L sodium heptanesulfonate (18∶41∶41) at a flow rate of 1 ml/min. The detection wavelength was set at 220 nm. Results Morphine showed a good linear correlation within the range 1.5~30.0 μg/ml ( r =0.9999)，The average recovery and RSD were 99.16％and 1.17％, respectively. Conclusions This method is simple, accurate and repeatable, which can be used for quality control of Yinghuan Powder.
MA Weiping,DENG Zili,and LI Lijun. Determination of morphine in Yinghuan Powder by HPLC[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2012, 23(6): 480-482.
