|
|
|
| Influence of venous leukocyte depletion on CD11/18 and CD14 leukocytes in canine Escherichia coli sepsis |
| CAI Zhiyang1,QIAN Baohua2,ZOU Yiqing3,WANG Liping4,and DENG Xiaoming5. |
| 1. Department of Anesthesiology, Hospital of Corps 8710, Chinese People’s Armed Police Forces, Putian 351133, China; 2. Department of Blood Transfusion, 5. Department of Anesthesiology, Changhai Hospital, Shanghai 200433,China; 3. Department of Anesthesiology, No. 476 Hospital of Fuzhou Military Command General Hospital of PLA, Fuzhou 350102, China; 4. Department of Anesthesiology, Fuzhou Military Command General Hospital of PLA, Fuzhou 350025, China |
|
|
|
|
Abstract Objective To study the influence of venous leukocyte depletion on CD11/18 and CD14 leukocytes in canine Escherichia coli sepsis. Methods Canine models of Escherichia coli sepsis were established by infusing the live Escherichia coli (O111∶B4) via vein. Eighteen hybrid dogs were anesthetized intravenously and randomly divided into two groups: the filtration group and the control group. The filtration group dogs underwent venous leukocyte depletion. T0′was set at the time of beginning infusion of the live Escherichia coli (O111∶B4), T0 was set at the time of beginning venous leukocyte depletion. Mean arterial pressure, pulse, body temperature, red blood cell concentration, platelet concentration, leukocyte concentration, TNF-α concentration, IL-6 concentration in serum, CD11/18 expression, and CD14 expression in both groups were measured at T0′, T0, T1 and T5. Results There were no significant differences in pulse, red blood cell concentration, platelet concentration, TNF-α concentration, and IL-6 concentration between the two groups. Mean arterial pressure at T1 in the filtration group was greater than that in the control group (P<0.05). The body temperature and leukocyte concentration at T1 in the filtration group was greater than that in the control group (P<0.05). CD11/18 expression of neutrophils at T5 in the filtration group was lower than that in the control group (P<0.05). CD14 expression of monocytes at T1, T5 in the filtration group was lower than that in the control group (P<0.05). Conclusions Venous leukocyte depletion improves canine hemodynamics, depresses leukocyte concentration, depresses the expression of the CD11/18 of neutrophils, CD14 of monocytes, whereas does not affect the inflammatory factor in serum.
|
|
Received: 20 September 2012
|
|
|
|
|
|
|
|
|
2013, Vol. 24 
