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| Alpha-crystallin promotes OECs survival in vitro |
| WANG Yanhua1,2,WANG Dongwu2,and YIN Zhengqin1. |
| 1. Department of Ophthalmology, General Hospital of Chinese People’s Liberation Army, Beijing 100853, China 2. The Hospital of Corps 8650, the Chinese People’s Armed Police Forces, Jinzhong 030600, China |
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Abstract Objective To investigate the effect of α-crystallin on the survival and proliferation of olfactory ensheating cells(OECs) in vitro. Methods OECs were cultured and treated with α-crystallin in DMEM/F12 or DMEM/F12 only. The number of surviving OECs was counte for after cultured 5days. The number of Brdu-labeled OECs was counted after Brdu was added in DMEM/F12. And cell cycle was analyzed by flow cytometry. Results α-crystallin promoted the survival of OECs at 6-13 days. The Brdu-labeled OECs in α-crystallin group was more in number than in control group. And the cell percentage of G2/M state and S state was higher in α-crystallin group than in control group. Conclusions α-crystallin promotes OECs survival and proliferation.
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Received: 27 April 2013
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2013, Vol. 24 
