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| Expression of IL-23/IL-17 axis in nasal polyp and its significance |
| DING Guochen1,CAO Zhiwei1,WANG Yahui2,and SI Ying3 |
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Abstract Objective To investigate the expression of interleukin-23(IL-23) and interleukin-17(IL-17) axis in the nasal polyp patients and explore the role of IL-23/IL-17 axis in the nasal polyp. Method Forty-five nasal polyp(NP) patients’s NP tissue and 30 simple deviation of nasal septum patients’ normal inferior turbinate tissue were collected. mRNA and protein expression of IL-23 and IL-17 were detected by immunohistochemistry and RT-PCR. SPSS 17.0 software package was used for statistical analysis. test and Spearman coefficient were used to analyse and compare the expression level and the correlation.Results RT-PCR demomstrated that the average expression level of IL-23p19mRNA and IL-17mRNA in NP tissues [(0.676±0.077),(0.681±0.044)] had significant differences (P<0.01) from those in normal inferior turbinate tissues [(0.212±0.015), (0.220±0.044)]. Immunohistochemical staining demomstrated that there were much more IL-23 and IL-17 positive cells in NP patients than in the normal controls.The average expression levels of IL-23 and IL-17 protein in NP tissues [(0.321±0.023), (0.315±0.023)] were significantly different (P<0.01) from those in normal inferior turbinate tissues [(0.199±0.011), (0.211±0.029)]. IL-17mRNA positively correlated with IL-23p19mRNA(r=0.723,P<0.01),and IL-17 protein positively correlated with IL-23 protein(r=0.991,P<0.01). Conclusion IL-23 and IL-17 may involved in the chronic inflammation of NP.IL-23 may be play a role through adjusting the level of IL-17.
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Received: 18 April 2013
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| [1] |
Bousquet J,Van Cauwenberge P,Khaltaev N,et al.Allergic rhinitis and its impact on asthma[J].J Allergy Clin Immunol,2001,108(5 Suppl):147-334.
|
| [2] |
韩德民,张 罗,黄 丹,等. 我国 11 个城市变应性鼻炎自报患病率调查[J]. 中华耳鼻咽喉头颈外科杂志,2007,42(5):378-384.
|
| [3] |
Kariyawasam H H, Rotiroti G. Allergic rhinitis, chronic rhinosinusitis and asthma: unravelling a complex relationship[J]. Curr Opin Otolaryngol Head Neck Surg,2013,21(1):79-86.
|
| [4] |
Pawankar R, Bunnag C, Khaltaev N, et al.Allergic Rhinitis and Its Impact on Asthma in Asia Pacific and the ARIA Update 2008[J]. World Allergy Organ J,2012,5(Suppl 3):S212-217.
|
| [5] |
中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组,中华医学会耳鼻咽喉科学分会鼻科学组.变应性鼻炎的诊断和治疗指南(2009,武夷山) [J].中华耳鼻咽喉头颈外科杂志,2009,44(12):977-978.
|
| [6] |
Strachan D. Hay fever,hygiene,and household size[J].BMJ,1989,299:1259-1260.
|
| [7] |
Jiang X D, Li G Y, Dong Z, et al. Correlation analysis of two serum-specific immunoglobulin E test systems and skin-prick test in allergic rhinitis patients from northeast China[J].Am J Rhinol Allergy, 2011,25(2):116-119.
|
| [8] |
Veskitkul J, Vichyanond P, Visitsunthorn N, et al. The development of allergic rhinitis in children previously diagnosed as nonallergic rhinitis[J]. Am J Rhinol Allergy,2013,27(1):43-47.
|
| [9] |
Eichner H.Present possibilities for diagnosis in human nasal secretions[J].Rhinology,1983,21(3):223-228.
|
| [10] |
Rondón C, Campo P, Galindo L, et al.Prevalence and clinical relevance of local allergic rhinitis[J].Allergy,2012,67(10):1282-1288.
|
| [11] |
张 罗,韩德民.解读2008年新版变应性鼻炎及其对哮喘的影响[J].中华耳鼻咽喉头颈外科杂志,2008,43(7):552-557.
|
| [12] |
张 罗,魏均民,韩德民.变应性鼻炎诊疗现状调查[J].中华耳鼻咽喉头颈外科杂志,2010,45(5):420-423.
|
| [13] |
尤少华,张 静,籍灵超,等. 变应性鼻炎特异性免疫治疗的疗效评价[J].国际耳鼻咽喉头颈外科杂志,2013,37(2):236-239.
|
| [14] |
Asher M I,Montefort S,Bjorksten B,et al.Worldwide time trends in the prevalence of symptoms of asthma,allergic rhinoconjunctivitis,and eczema in childhood:ISAAC Phases One and Three repeat multicountry cross-sectional surveys[J].Lancet,2006,368:733-743.
|
| [15] |
Kong W J,Chen J J,Zheng Z Y,et al.Prevalence of allergic rhinitis in 3-6-year-old children in Wnhan of China[J].Clin Exp Allergy,2009,39:869-874.
|
| [16] |
中华耳鼻咽喉头颈外科杂志编辑委员会鼻科组,中华医学会耳鼻咽喉头颈外科学分会鼻科学组、小儿学组,中华儿科杂志编辑委员会.儿童变应性鼻炎诊断和治疗指南(2010年,重庆)[J]. 中华耳鼻咽喉头颈外科杂志,2011,46(1):7-8.
|
| [1] |
. [J]. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(9): 802-803. |
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2013, Vol. 24 
