高原习服过程淋巴细胞线粒体DNA含量及氧化损伤变化规律

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Abstract Objective To study the changes in mitochondrial bioenergetics, mitochondrial DNA (mtDNA) and oxidative damage in leukocytes during acclimatization to highland hypoxia. Methods Twenty-seven lowlanders quickly entered plateau. Leukocyte mitochondrial membrane potential, 8-oxodG in mtDNA, mtDNA copies and PGC-1α protein expression were measured in plain and plateau at 3 d,7 d,90 d after entry. Results As compared with plain group, membrane potential was significantly decreased (P<0.05); 8-oxodG amounts, mtDNA copies and PGC-1α expression were markedly increased at 3 d and 7 d (P<0.05). And at 90 d, mtDNA copies and PGC-1α expression were significantly decreased (P<0.05). As compared with 7 d group, membrane potential was significantly increased (P<0.05); 8-oxodG amounts, mtDNA copies and PGC-1α expression were markedly decreased at 90 d (P<0.05). Conclusions In the early phase of acclimatization to highland, leukocyte bioenergetics remodeling mainly depends on increased mitochondrial content. In the later phase of acclimatization to highland, leukocyte bioenergetics remodeling mainly depends on elevated level of single mitochondrial health and energy metabolism.

Key words： acclimatization to high-altitude leukocytes mitochondrial DNA copy number oxidative stress

Received: 26 January 2014

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R446.11

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	Articles by authors
	BO Hai
	GUO Fengchun
	DUAN Fuqiang
	PENG Peng
	QIN Yongsheng

Cite this article:

BO Hai,GUO Fengchun,DUAN Fuqiang等. Changes in contents of leukocyte mitochondrial DNA and oxidative damage during acclimatization to highland[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2014, 25(8): 761-764.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2014/V25/I8/761

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