人脂肪来源间充质干细胞的制备及其质量检验方法

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Abstract Objective To study the human adipose derived mesenchymal stem cells in vitro amplification and quality inspection Methods and provide safe and qualified stem cells for clinical research. Methods Fifty human source liposuctioned fat, sheared, underwent trypsin and collagenase digestion, separation, culture, and according to the “stem cell-based medicinal product quality control of pre clinical research guiding principle” to detect the cell morphology, quantity, live rate, sterilization, mycoplasma, human source specific virus and swine virus, endotoxin, immunosuppressive activity, differentiation ability, immunophenotype detection and chromosome karyotype. Results Human adipose derived mesenchymal stem cell activity before cryopreservation is ≥92%, after revival≥82%; bacteria and endotoxin test negative, no internal and external source pathogenic bacteria; stem cell immunophenotype detection of CD90, CD105 positive, the positive rate>95%, CD34, CD45 and HLA-DR negative expression, the positive rate<2%; with osteogenic and adipogenic differentiation proficiency , it can inhibit the proliferation of allogeneic lymphocytes. Conclusions The preparation process and the standard of human adipose derived mesenchymal stem cell line according to the quality control standard of “stem cell-based medicinal product quality control of pre clinical research guiding principle”, it provides the experimental basis for the similar stem cell preparation and standardizing verification process.

Key words： adipose derived stem cells mesenchymal stem cells quality control immunophenotype differentiation karyotype analysis

Received: 20 November 2014

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	Articles by authors
	CHEN Chong
	YAN Junling
	LI Liang
	XU Xiao
	DING Hong
	TANG Suyang

Cite this article:

CHEN Chong,YAN Junling,LI Liang等. Human adipose derived mesenchymal stem cells and its quality test preparation[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2015, 26(2): 170-174.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2015/V26/I2/170

[1]	李辉, 李巧, 左振宇, 等. 人、大鼠和猪诱导多能干细胞染色体制备方法的建立和关键影响因素的分析[J]. 细胞生物学杂志, 2009, 31(3):443-447.
[2]	汤苏阳, 李彦, 闫俊灵, 等. 体外扩增的自体脂肪干细胞介导自体脂肪移植的临床研究[J]. 中国生物医药技术, 2013, 8(6): 433-438.
[6]	闫俊灵, 狄国虎, 丁红, 等. 脂肪干细胞介导的自体脂肪移植隆胸[J]. 中国组织工程研究, 2013, 17(5):878-885.
[7]	Zuk P A, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies[J]. Tissue Eng, 2001, 7(2):211-226.
[3]	国家药典委员会.中华人民共和国药典(2010年版) 三部[M]. 北京: 中国医药科技出版社, 2010: 附录95-98.
[8]	Salem H K, Thiemermann C. Mesenchymal stromal cells: current understanding and clinical status [J]. Stem Cells, 2010, 28(3): 585-596.
[4]	李梁, 贾德进, 闫俊灵, 等. 体外扩增自体脂肪干细胞对空鼻综合征患者粘膜功能重建的临床研究[J]. 解放军医学杂志, 2014, 51(10):1577-1580.
[5]	吴瑜, 冯建平, 林林, 等. STR 图谱分析法鉴别人源细胞交叉污染.中国生物制品学杂志, 2010, 23(1):74-86.
[9]	Chamberlain G, Fox J, Ashton B, et al. Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing [J]. Stem Cells, 2007, 25(11): 2739-2749.
[6]	闫俊灵, 狄国虎, 丁红, 等. 脂肪干细胞介导的自体脂肪移植隆胸[J]. 中国组织工程研究, 2013, 17(5):878-885.
[10]	Zavan B, Michelotto L, Lancerotto L, et al. Neural potential of a stem cell population in the adiposeand cutaneous tissues[J]. Neurol Res, 2010, 32(1):47-54.
[7]	Zuk P A, Zhu M, Mizuno H, et al. Multilineage cells from human adipose tissue: implications for cell-based therapies[J]. Tissue Eng, 2001, 7(2):211-226.
[11]	Lobna A. Aly. Efficiencyof systemic versus intralesional bone marrow-derived stem cells in regenerationof oral mucosa after induction of formocresol induced ulcers in Dogs[J]. DentRes J (Isfahan), 2014, 11(2):212-221.
[8]	Salem H K, Thiemermann C. Mesenchymal stromal cells: current understanding and clinical status [J]. Stem Cells, 2010, 28(3): 585-596.
[9]	Chamberlain G, Fox J, Ashton B, et al. Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing [J]. Stem Cells, 2007, 25(11): 2739-2749.
[10]	Zavan B, Michelotto L, Lancerotto L, et al. Neural potential of a stem cell population in the adiposeand cutaneous tissues[J]. Neurol Res, 2010, 32(1):47-54.
[11]	Lobna A. Aly. Efficiencyof systemic versus intralesional bone marrow-derived stem cells in regenerationof oral mucosa after induction of formocresol induced ulcers in Dogs[J]. DentRes J (Isfahan), 2014, 11(2):212-221.