小鼠尾悬吊法模拟微重力对骨代谢的影响

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Abstract Objective To study the effect of microgravity on the bone itself and metabolism.Methods 8-week-old widetype C57 mice were tail-suspended for 0, 2, 4, weeks. Related serum biochemical indices (Ca²⁺, ALP, BGP) were measured. Microstructure of bone was observed by micro-CT scanning. Three point bending test on the femurs by material testing machine helped to compare the changes of mechanical parameters. All the data were analysed.by SPSS18.0.Results Compared with control group, tail-suspended groups had a higher concentration in calcium in serum,the values of which were (2.134±0.08114) mmol/L, (2.261±0.07913) mmol/L, and (2.388±0.06112) mmol/L; lower levels of serum ALP and BGP, the difference was significant with a longer tail-suspension. The values of ALP was (4.598±0.4783) U/100 ml, (3.447±0.3930) U/100 ml, and (3.142±0.3280) U/100 ml; and the values of BGP were (8.529±0.5233) ng/ml, (8.108±0.3920) ng/ml, and (7.832±0.2821) ng/ml, which had statistically significant difference compared with control group (P<0.05).The scanning of femur showed obviously osteoporosis in tail-suspended mice. Compared with control group, number of bone trabecular reduced, the gap between them widened, thickness decreased simultaneously in the tail-suspended mice. Among which, BV/TV decreased most, the values of tailed-suspension for 2 and 4 weeks were 47.3% and 14.3% in the control group; There was a positive relationship between the osteoporosis and the time of tail-suspension. Mechanical parameters showed that the force decreased as well. Simulated microgravity promoted the loss of calcium in bone, mechanical properties of femurs of suspended-mice decreased, their capability of resistance to force decreasedas well. The forces to bone fracture in tailed-suspension for 0, 2 and 4 weeks were (6.220±0.482)mJ, (4.395±0.301)mJ, and (2.855±0.425)mJ, which had statistically significant difference compared with control group (P<0.05).Conclusions Simulated microgravity promotes the loss of calcium in bone, which can lead to osteoporosis, and the degree of that has a positive relationship to the time.

Key words： microgravity tail suspension osteoporosis mouse

Received: 11 June 2015

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	Articles by authors
	LI Yu
	ZHANG Xinhang
	ZHU Lei
	LI Jun
	and SUN Minlin

Cite this article:

LI Yu,ZHANG Xinhang,ZHU Lei等. Effect of microgravity simulated by mouse tail-suspension on bone[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2016, 27(4): 366-369.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2016/V27/I4/366

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