利用HaCaT细胞构建新型组织工程皮肤检测模型

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Abstract Objective To construct human immortalized keratinocytes HaCaT as seed cells for tissue engineering skin model.Methods A novel tissue engineered skin model was constructed for the detection of human immortalized keratinocytes and fibroblasts as seed cells. 10 kinds of chemicals were selected as test materials, the surface of local contact skin model was 15 min, and the relative viability of cells was measured by MTT colorimetry.Results Three different batches of the model, based on the relative values of cell viability as a decision, the new detection model constructed could determine eight kinds of harsh chemicals,after addition of IL-1α experiment improved the accuracy of the model, the accuracy rate was 75%.Conclusions This new model provides an effective in vitro test results and prove the feasibility of the model. At the same time it should use a greater number of substances for further validation and tests in different lab oratories to properly assess reproducibility.

Key words： HaCaT cells tissue-engineered skin fibroin irritant chemicals

Received: 22 November 2015

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R758.29

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	Articles by authors
	LI Yan
	ZHOU Zhoulin
	LU Tao
	and LI Ruixin

Cite this article:

LI Yan,ZHOU Zhoulin,LU Tao等. Construction of a new tissue engineering skin detection model using HaCaT cells[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2016, 27(7): 672-675.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2016/V27/I7/672

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