干预FoxM1表达对食管癌细胞增殖、周期及凋亡的影响

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Abstract Objective To investigate the effects of down-regulation of forkhead box protein M1 (FoxM1) expressions by shRNA on proliferation and apoptosis of esophageal squamous cell carcinoma (ESCC) cells.Methods Plasmid that highly expressed FoxM1-shRNA was successfully constructed and transfected with TE1 cell line. FoxM1 was analyzed by Western blot. Cell proliferation was tested by cell counting kit-8 while cell cycle and apoptosis were analyzed by flow cytometry and Annexin-V-PE/7-AAD staining kit.Results FoxM1 expressions in ESCC cells transfected with specific shRNA were significantly down-regulated at the protein level and cell proliferation was inhibited in a time-dependent manner. The inhibition rate was 68.0%±6.4% (P<0.05), with cell cycle arrested at G₁ phase arrest (59.14%±1.69% vs 40.51%±1.45%,t=14.23,P<0.01)and the apoptosis rate of TE1 cells reached 35.37% (2.48%±0.49% vs 35.37%±0.56%,t=76.56,P<0.01).Conclusions Interventions with FoxM1 gene expressions can inhibit the proliferation and apoptosis of ESCC cells, suggesting that FoxM1 can be a novel therapeutic target for ESCC.

Key words： esophageal squamous cell carcinoma forkhead box protein M1 gene silencing cell proliferation apoptosis

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	Articles by authors
	CHEN Jie
	YAN Xiaodi
	ZHANG Dongsheng
	QIAN Hongyun
	CHEN Ruijiao
	ZHOU Fangzheng

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CHEN Jie,YAN Xiaodi,ZHANG Dongsheng等. Effects of down-regulation of FoxM1 expressions on proliferation and apoptosis of esophageal squamous cell carcinoma cells[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2017, 28(10): 1035-1038.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2017/V28/I10/1035

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