原子力显微镜对结合HER-2后乳腺癌细胞膜的观察

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Abstract Objective To observe the morphological changes in the surface of breast cancer cell’s membrane with different expressions of human epidermal growth factor receptor-2 under the atomic force microscope(AFM)in order to provide a new method for improving the sensitivity and accuracy of HER-2 detection in breast cancer and stimulate new ideas for early diagnosis of tumor at the sub-cellular level.Methods Beast cancer cells of different histological types were divided into two groups based on their expression levels of human epidermal growth factor receptor-2: the positive group and negative group. The surface morphology of cells’ membrane was observed with atomic force microscopy respectively. Such characteristic morphological parameters as average roughness,mean peak-height,average maximum depth and surface area difference of these cells were quantitatively measured ,and the Results were statistically analyzed.Results There was significant difference between the two groups in cellular morphology. The surface of positive cell membrane of human epidermal growth factor receptor-2(HER-2) was rough and uneven, and the tuberositas was high and thin, but the tuberositas was heavy and wide in the HER-2 negative group.There were more tuberosities in the positive group than in the negative group in each count area.The difference in morphological characteristics between the two groups was obvious in the average roughness[(21.87±2.46)/(32.65±1.03),P＜0.000],mean peak-height[(13.94±1.01)/(31.15±3.89),P＜0.001], average maximum depth[(11.09±6.36)/(33.58±3.15),P＜0.001]and surface area[(6.27±2.03)/(19.65±1.13),P＜0.001].Conclusions The surface structure of cell membrane varies with different functional states, which may give us an insight into the mechanism, development and early diagnosis of cancer. It could also provide a new method for the diagnosis of the positive rate of HER-2.

Key words： atomic force microscopy breast cancer HER-2 cell membrane morphological characteristics

Received: 11 December 2016

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2017/V28/I8/799

[1]	Lapensee E W, Ben-Jonathan N. Novel roles of prolact in and estrogens in breast cancer resistance to chemotherapy [J]. En docrine-Related Cancer, 2010, 17: R91-R107.
[2]	付强,于世英,许三鹏.乳腺癌HER2过度表达预后相关因素研究[J].肿瘤防治研究,2008,35(3):14-16.
[3]	Binnig G,Rohrer H,Gerber C,et al.Surface studies by Scanning tunneling Microscope[J].Phys Rev Lett,1982,49:57-61.
[4]	Binnig G,Quate C F,Gerber C. Atomic force microscope[J].Phys Rev Lett,1986,56(9):930-933.
[5]	Tiwari D K,Tanaka S,Inouye Y,et al.Synthesis and Characterization of Anti-HER2 Antibody Conjugated CdSe/CdZnS Quantum Dots for Fluorescence Imaging of Breast Cancer Cell[J].Sensors(Basel),2009,9(11):9332-9364.
[6]	Cheng R,Meng F,Deng C,et al.Dual and multi-stimuli responsive poly meric-nanoparticles for program medsite specific drug delivery[J].Biomaterials,2013,34(14):3647-3657.
[7]	Gez, Lius. Functional block copolymer assemblies responsive totumor and intracel lular microenvironments for site-specific drug delivery and enhance dimaging performance[J].Chemical Society Reviews,2013,42( 17) :7289-7325.
[8]	《乳腺癌HER2检测指南》编写组.乳腺癌HER2检测指南[J].中华病理学杂志,2006,35(10):631-633.
[9]	Slamon D J,Clack G M,Wong S G,et al.Human breast cancer,correlation of relapse and survival with amplification of the HER-2 /neu oncogene[J].Sciene,1987,235:177-181.
[10]	乔杉杉,王大业.乳腺癌HER-2基因的研究进展及其靶点治疗[J].首都医科大学学报,2008,29(1):96-99.
[11]	刘尧,刘文超,陶玉荣.HER-2过表达与乳腺癌治疗的相关性研究进展[J].现代肿瘤医学,2010,8(1):182-184.
[12]	Ross J S,Fletcher J A.The HER-2/neu oncogene in breast cancer prognostic factor,predictive factor and target for therapy [J].Stem Cells,1998,16:413-428.
[13]	Brower F T,Ahmed S,Tartter P,et al.Prognostic variables in invasive breast cancer,contribution of comedo versus non-comedo in-situ component[J].Ann Surg Oncol,1995,2:440-444.
[14]	Cooke T,Reeves J,Lannigan A,et al.The value of the human epidermal growth factor receptor2(HER-2) as a prognostic marker[J].Eur J Cancer,2001,37(suppl 1):S3-S10.
[15]	Plodinec M, Loparic M, Monnier C A, et al. The nanomechanical signatureof breast canaer[J]. Nanotechnol, 2012, 7(11): 757-765.
[16]	Jin H, Pi J, Huang F, et al. BMP2 promotes migration and invasion of breast cancer cells via cytoskeletal reorganization and adhesion decrease: an AFM investigation[J].Appl-Microbiol Biotechnol,2012,2(93-94):1715-1723.
[17]	陈勇, 蔡继业. AFM对人乳腺癌细胞外纤连蛋白原纤维的形态学观察[J] .生物化学与生物物理学报, 2003, 35(8): 752-755.