运动预适应对急性低压低氧大鼠脑海马线粒体生物合成的影响

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Abstract Objective To investigate the effect of exercise preconditioning on mitochondrial biogenesis in the rat hippocampus after acute hypobaric hypoxia.Methods Rats were randomly divided into three groups: normal control group (NC), acute hypoxia group (AH), and exercise preconditioning+ acute hypoxia group (EP+AH). Rats in the EP+AH group underwent six weeks of treadmill training on the plain at a speed of 17 m/min, -5° grade for 60 min/day. Rats in AH and EP+AH groups were placed in a hypobaric hypoxia chamber at 0.06 MPa pressure and 10%±2% oxygen for 8 consecutive hours. The mtDNA copies, mitochondrial membrane potential, ROS generation rate, ATP synthesis activity, and the protein expression of PGC-1α, NRF-1, Tfam were measured.Results The mtDNA copies, ROS generation rate, PGC-1αprotein expression, and mitochondrial membrane potential in the AH group were 1.69±0.20, (9.49±1.25) pmol/(min·mg protein), 189.24±21.77, and 4.51±0.65 respectively, compared with 1.00±0.13, (4.83±0.66) pmol/(min·mg protein), 100.00±12.90, and 8.27±1.02 in the NC group respectively. The difference was statistically significant (P＜0.05 or P＜0.01) . The mtDNA copies, ROS generation rate, PGC-1αprotein expression, and mitochondrial membrane potential in the EP+AH group were 1.19±0.15, (6.01±0.93) pmol/(min·mg protein), 141.95±18.12, and 7.02±1.10, respectively, compared with 1.69±0.20, (9.49±1.25) pmol/(min·mg protein), 189.24±21.77, and 4.51±0.65 in the AH group respectively. The difference was also statistically significant (P＜0.05).Conclusions Exercise preconditioning can inhibit acute hypobaric hypoxia induced by mitochondrial biogenesis and oxidative stress while improving mitochondrial energy metabolism in the hippocampus.

Key words： exercise preconditioning hypoxia hippocampus mitochondrial biogenesis energy metabolism

Received: 12 July 2019

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	LIU Jing
	ZHANG Hongying
	BO Hai
	KANG Weimin

Cite this article:

LIU Jing,ZHANG Hongying,BO Hai等. Effect of exercise preconditioning on mitochondrial biogenesis in rat hippocampus after acute hypobaric hypoxia[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2019, 30(11): 932-935.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2019/V30/I11/932

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