车叶草苷对小鼠Lewis肺癌的抑瘤、抗炎作用及其机制

Abstract
Figure/Table
References
Related Citation (6)

Download: PDF (923 KB) HTML (1 KB)
Export: BibTeX | EndNote (RIS)

Abstract Objective To observe the anti-tumor and anti-inflammatory effect of asperuloside on mice with Lewis lung cancer, and to investigate the anti-inflammatory mechanism by which asperuloside inhibits the overactive tlr4/myd88/nf-κb pathway. Methods Thirty-six healthy c57bl/6j mice of SPF grade, after one week of adaptive feeding, underwent subcutaneous injection of Lewis cells under the armpits to establish a tumor-bearing model before they were randomly divided into the model control group, asperuloside group and cisplatin control group. Corresponding intervention was implemented, such as orally taking asperuloside or intraperitoneal injection of cisplatin, once a day for 3 weeks. After the last dose, the health status of these mice was observed, body mass and tumor mass measured, and their peripheral blood was collected. Levels of il-1β and tnf-α in serum were detected by ELISA. The expression levels of tlr4, myd88 and nf-κb in tumor tissues were measured with Western-blot technology. The localization and level of p-nf-κb in tumor tissues were measured by immunohistochemical technology. Results There was no significant difference in body weight between the three groups before administration. After administration, the weight of mice in the asperuloside group increased (P<0.01) compared with the model control group, and the difference was statistically significant. Compared with the asperuloside group, the weight of mice in the cisplatin control group was reduced (P<0.01) and the difference was also statistically significant. Compared with the model control group, the tumor index of mice in the asperuloside group and cisplatin control group was reduced (P< 0.01), and the difference was statistically significant. Compared with the model control group, the serum contents of il-1β and tnf-α were decreased(P<0.01), so were the expression levels of tlr4, myd 88, nf-κb and p- nf-κb in tumor tissues (P<0.01), and the difference was statistically significant. Conclusions Asperuloside can reduce inflammatory response in Lewis tumor- bearing mice.

Key words： asperuloside Lewis lung cancer anti-inflammatory tlr4/myd88/nf-κb signaling pathway

Received: 20 January 2020

ZTFLH:

R734.2

	Service

	E-mail this article
	Add to my bookshelf
	Add to citation manager
	E-mail Alert
	RSS
	Articles by authors
	CHU Weiwei
	YUAN Feiyang
	DING Guochen
	LV Wenjun
	SHI Changqian

Cite this article:

CHU Weiwei,YUAN Feiyang,DING Guochen等. Anti-tumor and anti-inflammatory effect of asperuloside on mice with Lewis lung cancer[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2020, 31(5): 401-404.

URL:

http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2020/V31/I5/401

[1]	洪庆,徐曼丽,汤建.车叶草苷的研究进展[J].中国野生植物资源,2018,37(4):43-45.
[2]	Yinghan Chan,Sin Wi,Joycelin Zhu, et al. Emerging therapeutic potential of the iridoid molecule, asperuloside: a snapshot of its underlying molecular mechanisms[J]. Chem Biol Interact,2020,315(5):1-10.
[3]	Liao Y,Xu L,Lin X,et al.Temporal trend in lung cancer burden attributed to ambient fine particulate matter in Guangzhou,China[J].Biomed Environ Sci,2017,30(10):708-717.
[4]	Sigismund S,Avanzato D,Lanzetti L.Emerging functions of the EGFR in cancer[J].Mol Oncol,2018,12(1):3-20.
[5]	Gazdar A F,Bunn P A,Minna J D.Small-cell lung cancer:what we know,what we need to know and the path forward[J].Nat Rev Cancer,2017,17(12): 725-737.
[6]	李红霞,杨磊,陈小丽,等.鸡矢藤环烯醚萜苷体外抗肿瘤活性研究[J].中国药师,2017,20(12):2117-2122.
[7]	冯原,江颖,周颖,等.补肺化瘀汤对气虚血瘀证Lewis肺癌小鼠的影响[J].中成药,2019,41(11):2625-2631.
[8]	He Jingyu,Lu Xianyuan,Wei Ting, et al. Asperuloside and asperulosidic acid exert an anti-inflammatory effect via suppression of the NF-κB and MAPK signaling pathways in LPS-Induced RAW 264.7 macrophages[J]. Int J Mol Sci, 2018,19(2027):1-12.
[9]	Qiu Jiaming,Chi Gefu,Wu Qianchao, et al.Pretreatment with the compound asperuloside decreases acute lung injury via inhibiting MAPK and NF-κB signaling in a murine model[J]. Int Immunopharmacol,2016,31(2):109-115.
[10]	程建超,张星星,童佳兵,等.芪玉三龙汤对肺癌小鼠肿瘤组织TLR4/MyD88/NF-κB通路分子表达的影响[J].中药药理与临床,2019,35(5):101-106.
[11]	杨静,周颖,金咏梅,等.胰腺癌患者循环肿瘤细胞及TLR4、TLR9、myd88的表达水平与其化疗效果及转移、复发的关系[J].现代生物医学进展,2017,17(28):5524-5527.
[12]	程建超,张星星,童佳兵,等.芪玉三龙汤对肺癌小鼠肿瘤组织TLR4/MyD88/NF-κB通路分子表达的影响[J].中药药理与临床,2019,35(5):101-106.
[13]	Zhou J,Deng Y,Li F,et al.Icariside Ⅱ attenuates lipopolysaccharide-induced neuroinflammation through inhibiting TLR4/MyD88/NF-κB pathway in rats[J].Biomed Pharmacother,2019,11(3):315-324.