流式细胞仪检测外周血淋巴细胞亚群的常见问题及其解决方案

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Abstract Objective To improve the accuracy of detection of peripheral blood lymphocyte subsets by flow cytometry. Methods Problems that were often encountered by our laboratory in the process of detecting peripheral blood lymphocyte subsets with the Mindray's BriCyte E6 flow cytometer in 2018 were summarized and analyzed, and corresponding solutions were proposed. Results The common problems encountered in the detection of peripheral blood lymphocyte subsets by flow cytometry included unclear clusters of scatter plots of the CD45-SSC gate, unclear classification clusters of scatter plots of the cross gate, inability to classify lymphocyte subsets, and false reduction of absolute lymphocyte subsets counting. The above problems could be solved by washing the blood samples with saline before sample preparation or by cleaning the samples with hemolysin before detection. Conclusions The problems encountered in the detection of peripheral blood lymphocyte subsets by Mindray BriCyte E6 flow cytometry can be solved by washing the blood samples with saline or cleaning the samples with hemolysin.

Key words： lymphocyte subsets scatter plot flow cytometer

Received: 05 February 2021

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	Articles by authors
	DUAN Cuijuan
	LING Aiqin
	ZHANG Cui
	WANG Ruiling
	SONG Na
	WANG Yufei

Cite this article:

DUAN Cuijuan,LING Aiqin,ZHANG Cui等. Solutions to problems with detection of peripheral blood lymphocyte subsets by flow cytometry[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2021, 32(10): 832-835.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2021/V32/I10/832

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