异丙酚对胶质瘤U87细胞增殖、侵袭、迁移及JAK2/STAT3通路的影响

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Abstract Objective To study the effects of propofol on the proliferation, invasion, migration and Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) pathway of glioma U87 cells. Methods MTT assay was used to determine the inhibitory effect of propofol at different concentrations on the growth of glioma U87 cells and human normal astrocyte HEB. Transwell invasion assay was used to determine the effects of 2 μM, 5 μM and 10 μM propofol on the invasive ability of glioma U87 cells in vitro. Scratch assay was used to determine the effects of 2 μM, 5 μM and 10 μM propofol on migration of glioma U87 cells in vitro, and the effect of propofol on JAK2/STAT3 pathway in U87 glioma cells was determined by Western blotting (WB). Results Compared with the control group, 5 μM, 10 μM, 25 μM, 50 μM and 100 μM propofol could significantly inhibit the proliferation of glioma U87 cells (P<0.05). Follow-up experiments were conducted with 2 μM, 5 μM and 10 μM propofol. Compared with the control group, the invasive ability of glioma U87 cells treated with 2 μM, 5 μM and 10 μM propofol decreased significantly (P<0.05). Scratch assay showed that the migration ability of U87 glioma cells treated with 2 μM, 5 μM and 10 μM propofol for 48 hours decreased by (19.69±2.67)%, (41.41±3.28)% and (59.75±2.91)% respectively, and there was significant difference between the two groups (P<0.05). The levels of JAK2 protein phosphorylation and STAT3 protein phosphorylation in U87 glioma cells treated with 2 μM, 5 μM and 10 μM propofol for 48 hours were significantly lower than those of the control group. Conclusions Propofol may inhibit the proliferation, invasion and migration of glioma U87 cells by down-regulating the expression of JAK2/STAT3 signal transduction pathway-related proteins.

Key words： propofol glioma cell proliferation cell invasion cell migration

Received: 20 November 2020

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	SU Kai
	YANG Yannan
	YANG Xingang

Cite this article:

SU Kai,YANG Yannan,YANG Xingang. Effects of propofol on proliferation, invasion, migration and JAK2/STAT3 pathway of glioma U87 cells[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2021, 32(3): 197-200.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2021/V32/I3/197

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