黄芩对妊娠晚期大鼠子宫运动节律及PGE、COX2水平的影响

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Abstract Objective To explore the effect of Scutellaria baicalensis on the uterine movement rhythm and levels of prostaglandin E (PGE) and cyclooxygenase 2 (COX2) in rats in the third trimester of pregnancy.Methods One hundred healthy adult SPF female rats and 50 male rats were adaptively reared for 7 days before mating in a 2∶1 male-female co-cage. The next day, female mice were smeared with sterile cotton swabs and examined by microscopy to find out whether they were pregnant. The forty pregnant rats were randomly divided into the control group and Scutellaria baicalensis low, middle and high dose groups in order of body weight, with 10 rats in each group. In the low, middle and high dose groups of Scutellaria baicalensis, doses that were 4,8 and 16 times (400 mg/kg, 800 mg/kg, 1600 mg/kg) the clinical equivalent dose for adults were given to the rats at the 19th day of pregnancy (once a day), while the control group was given the same dose of normal saline by gavage at the same time. The delivery of rat fetuses was observed on the same day. On the 21st day of pregnancy, the uterine activity, intensity of contractions and frequency of contractions in rats were recorded. The levels of PGE and COX2 mRNA and protein were detected by real-time PCR and Western blotting respectively.Results In the low, medium and high dose groups of Scutellaria baicalensis, the number of rats delivered was 36, 62 and 87 respectively, but was zero in the control group. The frequency of uterine contractions was (3.57±0.53, 5.82±0.87 and 5.82±0.87) times/min respectively, which was higher than that of the control group (2.11±0.31) times/min. The intensity of uterine contractions was (1.97±0.29, 2.58±0.38 and 3.26±0.50) g respectively, which was higher than that of the control group (1.06±0.23) g. The uterine activities were (6.85±1.03, 15.03±2.26 and 25.07±3.76) g/(time·min) respectively, which were higher than those of the control group (2.25±0.33) g/(time·min). The PGE mRNA levels in uterine muscle strip tissues were (1.26±0.19, 1.58±0.23 and 1.76±0.25) respectively, which were higher than in the control group (1.00±0.15). The COX2 mRNA levels were (1.33±0.19, 1.62±0.25 and 1.83±0.30) respectively, higher than those of the control group (1.01±0.16). The PGE protein levels were (0.59±0.05, 0.86±0.12 and 1.15±0.17) respectively, which were higher than those of the control group (0.27±0.05). The COX2 protein levels were (0.60±0.09, 0.93±0.14, 1.27±0.19) respectively, which were higher than those of the control group (0.37±0.06), and the above differences were all statistically significant (all P<0.05).Conclusions Scutellaria baicalensis may promote the synthesis and secretion of COX2 and PGE in uterine muscle tissues of rats during late pregnancy.

Key words： late pregnancy Scutellaria baicalensis uterine movement rhythm prostaglandin e cyclooxygenase-2

Received: 20 August 2021

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	ZHOU Jian
	QU Dongying
	YANG Qing

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ZHOU Jian,QU Dongying,YANG Qing. Effects of Scutellaria baicalensis on uterine movement rhythm and Pge, COX2 in rats during late pregnancy[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2022, 33(2): 149-153.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2022/V33/I2/149

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