青藤碱联合顺铂对顺铂耐药Hela细胞株增殖凋亡的作用

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Abstract Objective To explore the effect of sinomenine combined with cisplatin on proliferation and apoptosis of cisplatin-resistant cell lines.Methods Hela cells in the log phase were used to establish a cisplatin resistance model and divided into the control group, sinomenine group, activator group, and sinomenine combined with activator group. The control group was left untreated, sinomenine was added to the sinomenine group (40 μmol/L final concentration), the Notch signaling pathway ligand Jagged1 protein (500 ng/ml) to the activator group, and sinomenine (40 μmol/L final concentration) + Jagged1 protein (500 ng/ml) to the sinomenine combined with activator group. An MTT method was used to detect the proliferation-inhibiting rate of different concentrations of cisplatin in each group of cells. Flow cytometry was used to detect the rate of cell apoptosis. Western blot was used to detect the expressions of the cellular neurogenic gene Notch homologous protein 1 (Notch1), signal transduction and Transcription activator 3 (STAT3), and hair split-associated enhancer 1 (Hes1) protein.Results Compared with the control group, different concentrations of cisplatin in the sinomenine group increased the proliferation-inhibiting rate of cells, but IC50 was decreased. In the activator group, different concentrations of cisplatin reduced the proliferation-inhibiting rate of cells, but IC50 was increased (P<0.05). Compared with the sinomenine group, different concentrations of cisplatin in the sinomenine combined with activator group reduced the proliferation-inhibiting rate of cells but increased IC50 (P<0.05). Compared with the activator group, different concentrations of cisplatin in the sinomenine combined with activator group increased the inhibition rate of cell proliferation but decreased IC50 (P<0.05). Compared with the control group, the apoptosis rate in the sinomenine group was increased (P<0.05), but decreased in the activator group (P<0.05). Compared with the sinomenine group, the apoptosis rate in the sinomenine combined with activator group was increased (P<0.05). Compared with the activator group, the apoptosis rate in the sinomenine combined with activator group was increased (P<0.05). Compared with the control group, the expressions of Notch1, Stat3 and Hes1 in the sinomenine group were decreased (P<0.05), but increased in the activator group (P<0.05). Compared with the sinomenine group, the expressions of Notch1, Stat3 and Hes1 protein in the sinomenine combined with activator group were increased (P<0.05).Conclusions Sinomenine can increase the proliferation-inhibiting rate of chemotherapeutic drugs on cervical cancer cells and promote their apoptosis, thereby reducing the chemotherapy resistance of cervical cancer cells. This is possibly due to the inhibition of the Notch1/STAT3 signaling pathway.

Key words： sinomenine cervical cancer chemotherapy resistance apoptosis

Received: 12 July 2021

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R737.33

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	WANG Haiyan
	WANG Zhaohua
	MA Bo

Cite this article:

WANG Haiyan,WANG Zhaohua,MA Bo. Effects of sinomenine combined with cisplatin on proliferation and apoptosis of cisplatin-resistant cell lines[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2022, 33(2): 158-162.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2022/V33/I2/158

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