鸢尾素促进高糖环境中兔BMSCs骨向分化的机制

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Abstract Objective To explore irisin’s promotion mechanism of osteoblast differentiation of rabbit BMSCs in high glucose condition.Methods BMSCs of rabbit were divided into 4 groups: control, HG (high glucose), irisin (HG+irisin) and CQ (chloroquine; HG+irisin+CQ). After cultured in osteogenic differentiation medium for 5 days, western blotting was used to detect autophagy-related protein levels of P62 and LC3B; qRT-PCR was used to evaluate the expression levels of Runx2, ALP, COL-I and OCN; ALP activity was also tested.Results Compared with the control group, HG group significantly increased P62 and decreased LC3B-II protein levels, down-regulated mRNA expression of Runx2, ALP and COL-I, and reduced ALP activity (P＜0.05);however, irisin obviously decreased P62 and increased LC3B-II protein levels, up-regulated mRNA expression of Runx2, ALP and COL-I, and promoted ALP activity (P＜0.05); moreover, when inhibited autophagy with CQ, the effect of irisin was blunted (P＜0.05).Conclusion Irisin promotes osteoblast differentiation of BMSCs in high glucose condition via activating autophagy.

Key words： irisin autophagy BMSCs osteoblast

Received: 01 March 2022

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	Articles by authors
	SHI Zhiyun
	ZOU Rongfang
	SHI Quan
	CHEN Fei
	ZHAI Yujie
	HUANG Yang

Cite this article:

SHI Zhiyun,ZOU Rongfang,SHI Quan等. Irisin’s promotion mechanism of osteoblast differentiation of rabbit BMSCs in high glucose condition[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2022, 33(7): 560-563.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2022/V33/I7/560

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