鸢尾素对炎症状态下小鼠骨髓间充质干细胞成骨分化的影响

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Abstract Objective To investigate the effects of irisin on osteoblast differentiation of bone-marrow mesenchymal stem cells (BMSCs) in the inflammatory microenvironment.Methods BMSCs were isolated, cultured and divided into 3 groups: Vehicle, LPS and Irisin (LPS plus irisin intervention). CCK-8 was used to access cell proliferation. After cultured in osteogenic differentiation medium, ALP activity was evaluated, mRNA levels of Runx2, Osx, ALP and OCN and protein levels of ALP and OCN were also investigated. Moreover, alizarin red was used to determine mineralization level.Results Compared with the Vehicle group, LPS significantly promoted BMSCs proliferation (P＜0.05); LPS downregulated Runx2, Osx, ALP and OCN mRNA expression and reduced ALP and OCN protein levels, while irisin intervention could obviously upregulate Runx2, Osx and ALP mRNA expression, enhanced ALP and OCN protein levels, and raised mineralization level (P＜0.05). Conclusions Irisin can promote osteoblast differentiation of BMSCs in the inflammatory microenvironment.

Key words： irisin inflammatory microenvironment bone-marrow mesenchymal stem cells osteoblast differentiation

Received: 21 March 2022

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	Articles by authors
	GUO Shuqin
	WEN Chunfang
	ZHU Biao
	TIAN Xiaoyu
	DANG Ruijie
	WANG Lijun

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GUO Shuqin,WEN Chunfang,ZHU Biao等. Effects of irisin on osteoblast differentiation of bone-marrow mesenchymal stem cells derived from mice in inflammatory condition[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2023, 34(2): 140-143.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2023/V34/I2/140

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