髓源性生长因子对高糖环境BMSCs成骨分化的影响及其机制

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Abstract Objective To examine the effect of myeloid-derived growth factors (MYDGF) on the osteogenic differentiation of rat bone marrow mesenchymal stem cells (BMSCs) in high glucose environment and its molecular mechanism. Methods BMSCs were isolated and cultured in vitro, the surface markers of BMSCs were detected by flow cytometry, the osteogenic status of BMSCs was quantitatively detected by alkaline phosphatase (ALP), and the mRNA levels of OSX, RUNX2 and COL-I were detected by RT-qPCR. The protein levels of p-GSK3β and β-catenin were detected by western blot. Results ALP activity and mRNA expression of OSX, RUNX2 and COL-I in BMSCs were inhibited by high glucose (P＜0.05), and the inhibitory effect was improved after adding MYDGF (P＜0.05). The inhibitory effect of high glucose on the expression of p-GSK3β and β-catenin could be reversed by MYDGF [p-GSK3β:(0.85±0.13) vs. (0.15±0.07), β-catenin: (0.79±0.14) vs. (0.37±0.11); P＜0.05], and the promotion of MYDGF on the expression of p-GSK3β and β-catenin were inhibited after DKK1(the inhibitor of Wnt signaling pathway) treatment [p-GSK3β:(0.43±0.11) vs. (0.85±0.13), β-catenin:(0.20±0.06) vs. (0.79±0.14); P＜0.05]. Conclusions MYDGF may promote the osteogenic differentiation of BMSCs in high glucose environment by activating the Wnt/β-catenin signaling pathway.

Key words： myeloid-derived growth factor bone marrow mesenchymal stem cells high glucose osteogenic differentiation Wnt/β-catenin signaling pathway

Received: 17 October 2022

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R318.08

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	Articles by authors
	ZHAO Xiqiang
	LU Wenhua
	SHANG Liqiang
	DANG Ruijie
	AN Ran
	HE Xin

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ZHAO Xiqiang,LU Wenhua,SHANG Liqiang等. Effect of myeloid-derived growth factors on osteogenic differentiation of BMSCs in high glucose environment and its mechanism[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2023, 34(6): 507-510.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2023/V34/I6/507

[1]	Laakso M, Fernandes S L. Genetics of type 2 diabetes: past, present, and future[J]. Nutrients,2022,14(15):3201.
[2]	Sheng C, Guo Y, Hou W, et al. The effect of insulin and kruppel like factor 10 on osteoblasts in the dental implant osseointegration in diabetes mellitus patients[J]. Bioengineered, 2022, 13(6): 14259-14269.
[3]	Tang D, Wang E, Xu Y, et al. Is hyperglycemia the only risk factor for implant in type 2 diabetics during the healing period [J]. Oral Dis, 2021, 27(6):1551-1563.
[4]	Wang P, Mariman E, Keijer J, et al. Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines[J]. Cell Mol Life Sci, 2004, 61(18):2405-2417.
[5]	Korf-Klingebiel M, Reboll M R, Klede S, et al. Myeloid-derived growth factor (C19orf10) mediates cardiac repair following myocardial infarction[J]. Nat Med, 2015, 21(2):140-149.
[6]	Wang L, Li Y, Guo B, et al. Myeloid-derived growth factor promotes intestinal glucagon-like peptide-1 production in male mice with type 2 diabetes[J]. Endocrinology, 2020, 161(2):1-19.
[7]	Xu X, Li Y, Shi L, et al. Myeloid-derived growth factor (MYDGF) protects bone mass through inhibiting osteoclastogenesis and promoting osteoblast differentiation[J]. EMBO Rep, 2022, 23(3): e53509.
[8]	刘翠, 徐晓丽, 李金儒,等. 髓源性生长因子对糖尿病小鼠BMSCs骨向分化的影响及机制研究[J]. 实用口腔医学杂志, 2020, 36(6):865-869.
[9]	李金儒, 位朵, 甄国朋, 等. Irisin对高糖环境中BMSCs骨向分化的影响及机制研究[J].华南国防医学杂志, 2019, 33(7):445-449, 472.
[10]	Tian Z, Zhang X, Zhao Z, et al. The Wnt/β-catenin signaling pathway affects the distribution of cytoskeletal proteins in Aβ treated PC12 cells[J]. J Integr Neurosci, 2019, 18(3):309-312.
[11]	Monje A, Catena A, Borgnakke W S. Association between diabetes mellitus/hyperglycaemia and peri-implant diseases: systematic review and meta-analysis[J]. J Clin Periodontol, 2017, 44(6): 636-648.
[12]	Camargo W A, de Vries R, van Luijk J, et al. Diabetes mellitus and bone regeneration: a systematic review and meta-analysis of animal studies[J]. Tissue Eng Part B Rev, 2017, 23(5): 471-479.
[13]	Fayed A, Nokeety M M, Heikal A A, et al. Fibroblast growth factor-23 is a strong predictor of insulin resistance among chronic kidney disease patients[J]. Ren Fail, 2018, 40(1):226-230.
[14]	Su T, Xiao Y, Xiao Y, et al. Bone marrow mesenchymal stem cells-derived exosomal MiR-29b-3p regulates aging-associated insulin resistance[J]. ACS Nano, 2019, 13(2):2450-2462.
[15]	张玉, 王艳, 赵然尊, 等. 骨髓源性生长因子对高糖诱导H9c2细胞焦亡的改善作用[J].第三军医大学学报, 2021, 43(10): 923-929.
[16]	Wei B, Wang W, Liu X, et al. Gelatin methacrylate hydrogel scaffold carrying resveratrol-loaded solid lipid nanoparticles for enhancement of osteogenic differentiation of BMSCs and effective bone regeneration[J]. Regen Biomater, 2021, 8(5):1-14.
[17]	Wang X, Wang Y, Gou W, et al. Role of mesenchymal stem cells in bone regeneration and fracture repair: a review[J]. Int Orthop, 2013, 37(12):2491-2498.
[18]	Zhang P, Zhang H, Lin J, et al. Insulin impedes osteogenesis of BMSCs by inhibiting autophagy and promoting premature senescence via the TGF-β1 pathway[J]. Aging (Albany NY),2020,12(3): 2084-2100.
[19]	Majidinia M, Sadeghpour A, Yousefi B. The roles of signaling pathways in bone repair and regeneration[J]. J Cell Physiol, 2018, 233(4): 2937-2948.
[20]	Lai K, Xi Y, Du X, et al. Activation of Nell-1 in BMSC Sheet promotes implant osseointegration through regulating Runx2/Osterix axis[J]. Front Cell Dev Biol, 2020, 8:868.
[21]	Dong M, Jiao G, Liu H, et al. Biological silicon stimulates collagen type 1 and osteocalcin synthesis in human osteoblast-like cells through the BMP-2/Smad/RUNX2 signaling pathway[J]. Biol Trace Elem Res, 2016, 173(2):306-315.
[22]	Choi R B, Robling A G. The Wnt pathway: an important control mechanism in bone's response to mechanical loading[J]. Bone, 2021, 153:116087.
[23]	Yu F, Yu C, Li F, et al. Wnt/β-catenin signaling in cancers and targeted therapies[J]. Signal Transduct Target Ther, 2021, 6(1): 307.
[24]	Zhang B, Liu N, Shi H, et al. High glucose microenvironments inhibit the proliferation and migration of bone mesenchymal stem cells by activating GSK3 beta[J]. J Bone Miner Metab, 2016, 34(2):140-150.
[25]	Deng Y, Zhu W, Lin A, et al. Exendin-4 promotes bone formation in diabetic states via HDAC1-Wnt/ β-catenin axis[J]. Biochem Biophys Res Commun, 2021, 544:8-14.