髓源性生长因子对2型糖尿病小鼠胰岛素抵抗及肝脏炎性反应的影响

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Abstract Objective To investigate the effects of myeloid-derived growth factor (MYDGF) on insulin resistance and inflammatory response of liver from type 2 diabetic mice. Methods C57 mice were randomly divided into Vehicle group, DM group and MYDGF group. After administration for 12 weeks, fasting blood glucose (FBG), insulin and HOMA-IR were determined. Tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in serum as well as superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and Catalase in the liver were measured. Morphological changes of liver were assessed by HE staining. Phosphorylation levels of IKKβ and IkBα were detected by Western blot. Results Compared with DM group, the levels of FBG[(6.91±0.71) mmol/L vs. (19.46±1.12) mmol/L], and insulin in MYDGF group[(13.29±1.42) Mu/L vs. (21.88±1.58) Mu/L] and HOMA-IR[(4.08±0.65) vs. (18.90±1.37)] obviously decreased (P<0.05). The concentrations of TNF-α[(32.17±3.18)ng/L vs. (66.39±4.51) ng/L] and IL-6 [(13.65±0.89) ng/L vs. (21.55±3.27) ng/L] also significantly decreased (P<0.05). Liver SOD [(297.54±20.43) U/mg vs. (198.32±19.29) U/mg], GSH-Px[(0.65±0.05) U/mg vs. (0.19±0.04) U/mg] and Catalase [(220.34±19.82) U/mg vs. (134.69±18.81) U/mg] levels increased (P<0.05); in addition, inflammatory cell infiltration and hepatocyte steatosis attenuated; lastly, phosphorylation levels of IKKβ [p-IKKβ:IKKβ, (0.97±0.10) vs. (1.39±0.11)] and IkBα [p-IκBα:IκBα, (0.93±0.07) vs. (1.44±0.06)] significantly increased (P＜0.05) in MYDGF group. Conclusions MYDGF improves insulin resistance, which may be attributed to attenuated inflammatory response from diabetic liver via activating the IKKβ/IkBα signaling pathway.

Key words： myeloid-derived growth factor IKKβ/IkBα signaling pathway type 2 diabetes insulin resistance

Received: 16 October 2023

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	SU Fang
	FU Zhiguang
	FANG Wencan
	WANG Ying
	HE Mingjuan
	ZHANG Yongbin

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SU Fang,FU Zhiguang,FANG Wencan等. Effects of myeloid-derived growth factor on insulin resistance and inflammatory response of liver from type 2 diabetic mice[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2024, 35(3): 201-204.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2024/V35/I3/201

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