超声引导下急性缺血性左室舒张功能障碍并二尖瓣反流犬模型的构建

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Abstract Objective To establish the model of acute ischaemic left heart failure with mitral regurgitation in beagles. Methods (1)The construction of mitral regurgitation (MR)animal model: a catheter was introduced into left ventricle through the carotid artery in 15 experiment beagles, then a biopsy clamp was put into the mitral valve nearby through the catheterization and chordae tendineae was pinched under the guidance of echo in order to construct mitral regurgitation.(2) The construction of acute ischaemic left ventricular (LV) diastolic heart failure: 15 beagles with MR were further processed with coronary microembolization by microspheres repeatedly. Results (1) All mitral regurgitation models were successfully produced. The chordae tendineaes were broken in thirteen dogs, causing moderate MR. One dog with natural mild MR was with broken chordae tendineae. Continuous wave Doppler showed that the MR peak velocity was (483±1.12) cm/s, the maximun pressure gradient was (162.7±1.8) mmHg. (2)12 models of acute ischaemic left diastolic heart failure with mitral regurgitation were established. Left ventricular end-diastolic pressure emitting equal or greater than 5 mmHg was defined as model successful. The left ventricular end-diastolic pressure (LVEDP), -dP/dtmax, Tau-c in successful model were increased significantly (P＜0.05). Conclusions This method is safe, feasible and easily successful in constructing the MR animal model under the guidance of transthoracic echocardiography.Coronary microembolization with microspheres is a sueeessfull way for establishing the acute ischaemic left diastolic heart failure.

Key words： guiding of transthoracic echocardiography MR acute myocardial ischaemic left ventricular (LV) diastolic failure beagles animal model

Received: 20 May 2016

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	Articles by authors
	SUN Jing
	FAN Chunzhi
	DOU Jianping
	and WEN Chaoyang

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SUN Jing,FAN Chunzhi,DOU Jianping等. Establishing the model of acute ischaemic left heart failure with mitral regurgitation in beagles[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2016, 27(9): 906-909.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2016/V27/I9/906

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