IFN-γ预处理骨髓间充质干细胞及其抑制小胶质细胞 活化作用的影响

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Abstract Objective To explore the inhibitory effect of pre-treatment of one marrow mesenchymal stem cells (BMMSCs) with interferon-γ and IFN-γ on the activation of microglia(MG) induced by lipopolysaccharide(LPS).Methods BMMSCs were isolated and cultured using the whole bone marrow adherence method. Then, 100ng/ml IFN-γ was used to induce the activation of BMMSCs and10μg/ml lipopolysaccharide was used as the inflammatory stimulating factor to induce the activation of microglia. After isolation and purification, microglia were seeded in 6-well plates at the density of 1×10⁵/well and BMMSCs were co-cultured with microglia with the cell contact cell method for 24 h in the following groups: B group(BMMSCs group), L+B group(LPS+BMMSCs group), I+B group(IFN-γ pretreatment BMMSCs group), L+I+B group(LPS+IFN-γ pretreatment BMMSCs group), M group(microglia group), L+M group(LPS+ microglia group), B+L+M group(BMMSCs+ LPS+ microglia group), and I+B+L+M group(BMMSCs pretreated with IFN-γ+LPS+ microglia group). 24 hours later, the expressions of TNF-α、IL-1βin cell culture supernatants were detected by ELISA kit and the expression of microglia surface marker CD68 was detected by immunofluorescence.Results In the L+M group,LPS promoted the expression of CD68 and the production of TNF-α and IL-1β in microglial cells. The expression of CD68 and the production of TNF-α and IL-1β in B+L+M group and I+B+L+M group were lower than in L+M group, and there was statistically significant difference between the B+L+M group and the I+B+L+M group(P＜0.05).Conclusions Both BMMSCs and IFN-γ pretreatment of BMMSCs can inhibit the activation of LPS-induced microglia by direct cell contact. After IFN-γ pretreatment ,BMMSCs could more strongly inhibit the activity of microglia.

Key words： bone marrow mesenchymal stem cells microglia activation pretreatment IFN-γ

Received: 22 February 2017

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R329.28

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	Articles by authors
	DU Chunyan
	YANG Jing
	WANG Xiaochen
	FENG Zeguo
	GUO Quanyi
	and DAI Xin

Cite this article:

DU Chunyan,YANG Jing,WANG Xiaochen等. Inhibitory effects of IFN-γ pretreated bone marrow mesenchymal stem cells on lipopolysaccharide-induced microglia activation[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2017, 28(6): 600-604.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2017/V28/I6/600

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