高压氧对缺氧缺血性脑损伤新生大鼠脑皮质线粒体生物合成的影响

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Abstract Objective To observe the effect of hyperbaric oxygenation on mitochondrial biogenesis and related signal pathways in the cerebral cortex of neonatal rats after hypoxic ischemic brain damage.Methods Forty-eight 7-day postnatal Wistar rats were randomly divided into 3 groups: sham operation group (Sham, n=16), hypoxic-ischemic brain damage group (HIBD,n=16) and hyperbaric oxygenation treated group (HBO+HIBD, n=16). An HIBD model was established via ligation of the right common carotid artery followed by hypoxia exposure (8%O₂+92%N₂) for two hours. In HBO+HIBD group, rats received hyperbaric oxygenation (90 min a day, for 7 consecutive days) after HIBD.Eight rats in each group were sacrificed at 24 h after HBO, and the cerebral cortex was used to detect the expressions of COXⅣ, PGC-1α, Tfam and NRF1. Other rats were tested with Morris water maze at 40 days old.Results As compared with HIBD group, the escape latency in HBO+HIBD group was obviously shorter(21.49±3.77 vs 42.50±6.46,P<0.01). The number of times of crossing the platform and the expressions of COXⅣ, PGC-1α, Tfam, and NRF1 were significantly increased (7.41±1.13 vs 5.26±0.78, 82.15±14.84 vs 62.83±11.02, 112.19±18.41 vs 67.26±10.40, 92.15±13.99 vs 76.40±9.32, and 72.29±12.88 vs 49.28±7.14, respectively, P<0.05~0.01).Conclusions Hyperbaric oxygenation can ameliorate spatial learning and memory impairment in neonatal rats after HIBD. The protective mechanism of HBO may be related to the promotion of PGC-1a-NRF1/Tfam mediated mitochondrial biogenesis in the cerebral cortex.

Key words： hyperbaric oxygenation neonatal rats hypoxic ischemic brain damage PGC-1 mitochondrial biogenesis

Received: 12 February 2018

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	JING Cuicui
	ZHANG Ying
	ZHOU Muo
	LIU Yuanyuan
	ZHANG Hongying

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JING Cuicui,ZHANG Ying,ZHOU Muo等. Hyperbaric oxygenation promotes mitochondrial biogenesis in cerebral cortex of neonatal rats after hypoxic ischemic brain damage[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2018, 29(8): 786-789.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2018/V29/I8/786

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