人型结核分枝杆菌H37Ra在构建实验性自身免疫性重症肌无力大鼠模型中的应用

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Abstract Objective To investigate the role of mycobacterium tuberculosis H37Ra in the establishment of myasthenia gravis animal models with rat acetylcholine receptor alpha subunit 97-116 peptide (R97-116) as an immunogen.Methods Subcutaneous inoculation was carried out in the foot pad and skin of Lewis rats with R97-116 as the immunogen. Twenty rats were divided into the experimental group and control group according to whether H37Ra was added, and a blank control group was set up at the same time. The difference of behavior, body weight and antibody titers of acetylcholine receptors between the three groups was compared.Results In the experimental group, local inflammation was obvious,three rats died, six showed asthenia and positive antibodies, and one showed no obvious abnormal manifestations. In the control group, local inflammatory reactions were also present, and only two showed symptoms of mild weakness, one of which was suspected of having positive antibodies and the other one was negative. The weight of the experimental group [(190.21±8.35)g] was significantly lower than that of the control group [(243. 22±7.98)g] and the blank control group[(251.22±5.81)g], and the difference was statistically significant (P<0.05), but there was no statistically significant difference between the control group and the blank control group.Conclusion H37Ra is a necessary reagent for the success of this experiment.

Key words： myasthenia gravis animal model mycobacterium tuberculosis H37Ra

Received: 02 January 2019

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2019/V30/I5/391

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