角叉菜胶构建大鼠慢性非细菌性前列腺炎模型的影响因素

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Abstract Objective To explore the influencing factors of carrageenan onchronic nonbacterial prostatitis (CNP) modelsin rats. Methods Adult male rats were injected with carrageenan to make models, and the effects of mouse species (SD rats and Wistar rats), injection sites (seminal vesicle andventral lobe of prostate) and injection concentration (1% and 3%) on the modeling were observed.The modeling effect was determined according to the results of HE stainingof prostate histopathological indicators. Results Compared with Wistar male rats, the results of HE staining in SD male prostate tissues were significantly higher (P<0.05). Spontaneous inflammation appeared in parts of normal Wistar male rats (P<0.05), which mightaffect the observation of later experimental results. The effect of bilateral seminal vesicle injection was better than that of left and right ventral prostatic injection(P<0.05), but it increased the mortalityof rats and acute inflammatory reaction occurred; compared with left and right ventral prostaticinjection, the successful rate of model construction was higher, and the local multi-point injection method could make up for the lackof small infiltration area.The modeling effect of 3% carrageenan saline solution was more significant than that of 1% carrageenan saline solution (P<0.05). Conclusions SD male rats are more sensitive to carrageenan than Wistar male rats. A stable and ideal CNP model will be successfully established after 21 days of local injection of 3% carrageenan saline solution into the left and right ventral lobe of the prostate of SD male rats.

Key words： chronic prostatitis male rats animal model carrageenan model evaluation

Received: 04 May 2023

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	Articles by authors
	SU Xuzhen
	ZHANG Qiang
	XIANG Ruijun
	ZHANG Chunlei
	ZHANG Bin
	andCHANG Dehui

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SU Xuzhen,ZHANG Qiang,XIANG Ruijun等. Influencing factors of carrageenan to establish rat models of chronic nonbacterial prostatitis[J]. Med. J. Chin. Peop. Armed Poli. Forc., 2023, 34(10): 833-836.

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http://journal08.magtechjournal.com/Jwk_wjyx/EN/ OR http://journal08.magtechjournal.com/Jwk_wjyx/EN/Y2023/V34/I10/833

[1]	Li Z, Liu J, Liu P, et al. Effects of electroacupuncture with different waveforms on chronic prostatitis/chronic pelvic pain syndromes: a randomized controlled trial[J]. Contrast media mol imaging,2022,2022:6866000.
[2]	AlKhayal A, Alasmari F A, Alomeyr B K, et al. Chronic pelvic pain syndrome symptoms significantly affect medical students' well-being[J]. Urol Ann, 2022,14(3):259-264.
[3]	Evans-Durán B, Tripp D A, Campbell J, et al. Chronic prostatitis/chronic pelvic pain syndrome-related pain symptoms and their impact on sexual functioning[J]. Can Urol Assoc J,2022,16(6):222-227.
[4]	Cyril A C, Jan R K, Radhakrishnan R. Pain in chronic prostatitis and the role of ion channels: a brief overview[J]. Br J Pain,2022,16(1):50-59.
[5]	戴应和,龙小琴,刘晨琪,等.角叉菜胶诱导慢性非细菌性前列腺炎动物模型的制备与评价[J].中国实验动物学报,2017,25(5):544-550.
[6]	朱佩轩,范琼尹,张晶璇,等.角叉菜胶致大鼠慢性前列腺炎模型的影响因素[J].世界中医药,2021,16(14):2094-2100.
[7]	王先进,钟山,张存明,等.大鼠慢性非细菌性前列腺炎模型的建立及病理组织学特征[J].中华泌尿外科杂志,2012(4):282-287.
[8]	Radhakrishnan R, Nallu R S. Development and characterisation of a novel animal model of prostate inflammation-induced chronic pelvic pain[J]. Inflammopharmacology,2009,17(1):23-28.
[9]	Chen R Z, Cui L, Guo Y J, et al. In vivo study of four preparative extracts of cematis terniflora DC. for antinociceptive activity and anti-inflammatory activity in rat model of carrageenan-induced chronic non-bacterial prostatitis[J]. J Ethnopharmacol,2011,134(3):1018-23.
[10]	Wang W, Chen R, Wang J. Procyanidin B2 ameliorates carrageenan-induced chronic nonbacterial prostatitis in rats via anti-inflammatory and activation of the Nrf2 pathway[J]. Biochem Biophys Res Commun,2017,493(1):794-799.
[11]	Zeng F, Chen H, Yang J, et al. Development and validation of an animal model of prostate inflammation-induced chronic pelvic pain: evaluating from inflammation of the prostate to pain behavioral modifications[J]. PLoS One,2014, 9(5):e96824.
[12]	Chen C S, Chang P J, Lin WY, et al. Evidences of the inflammasome pathway in chronic prostatitis and chronic pelvic pain syndrome in an animal model[J]. Prostate,2013,73(4):391-7.
[13]	董德刚,宋梅,易军,等.慢性非细菌性前列腺炎大鼠前列腺线粒体损伤及解毒活血汤干预作用研究[J].中华男科学杂志,2021,27(6):483-488.
[14]	朱治亚,吴泳蓉,闵杰,等.麝香配伍乳香对慢性前列腺炎模型大鼠脊髓L5-S2段背角P物质表达水平的影响[J].中国性科学,2021,30(2):112-116.
[15]	张春玲,范顺明,李星,等.知母黄柏盐制前后组成滋肾丸对慢性前列腺炎及肠道菌群的对比研究[J].中药药理与临床,2021,37(1):34-40.
[16]	Hajighorbani M, Ahmadi-Hamedani M, Shahab E, et al. Evaluation of the protective effect of pentoxifylline on carrageenan-induced chronic non-bacterial prostatitis in rats[J]. Inflammopharmacology,2017,25(3):343-350.
[17]	Sutulovic N, Grubac Z, Suvakov S, et al. Chronic prostatitis/chronic pelvic pain syndrome increases susceptibility to seizures in rats and alters brain levels of IL-1β and IL-6[J]. Epilepsy Res,2019,153:19-27.
[18]	朱宇狄,林宗明,杨春欣.清前方对实验性慢性非细菌性前列腺炎的作用[J].现代泌尿外科杂志,2007,(6):395-397.
[19]	宫傲磊,陈东,王那钦,等.益气通络排浊颗粒对慢性非细菌性前列腺炎大鼠锌离子、超氧化物歧化酶水平的影响[J].中华中医药学刊,2022,40(6):66-69.
[20]	王文地,郑玉婷,于冰莉,等.慢性前列腺炎模型筛选及其肾功能评价[J].西北药学杂志,2021,36(2):234-238.

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