Differential proteomics analysis on osteoblasts stimulated by mechanical strain
LI Bin1,ZHANG Xizheng2,ZHU Xu3,and GAO Hao1
1. Second Department of Surgery, 3. Department of Respiratory Medicine, Yunnan Provincial Corps Hospital, Chinese People’s Armed Police Forces, Kunming 650111, China; 2.Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin 300161, China
Abstract：Objective To study proteomic differential expressions of mouse osteoblasts stimulated by mechanical strain. Methods Mouse osteoblasts were randomly divided into two groups:experimental group were subjected to mechanical strain of 0.5 Hz, 2000 με for 6 hours by using four-point-bend loading system;control group did not undergo any stress. Proteins extracted from cells were separated by two-dimensional electrophoresis ( 2-DE), and the differentially expressed protein spots were analysed. Results A total of( 533±14)and( 512±11) protein spots were resolved by 2-DE of experimental and control groups extractions , respectively.Nine protein points displayed significant changes in strain treated osteoblasts, 7 increased in expression and 2 decreased. Conclusions The osteoblasts can express differential proteins stimulated by mechanical strains and these proteins may play an important role in molecular mechanism of osteoblasts under mechanical strain loading.
2013, Vol. 24 
