Abstract：Objective To verify the effect of high-resolution microendoscopic imaging and to compare the effects of different pretreatment methods for colon polyps specimens in high-resolution microendoscopic imaging and the accuracy of the diagnosis of disease. Methods In vitro specimens from 90 patients with colon polyps were randomly divided into three groups: using saline (group A), saline plus mucus removing agent (group B), saline, and mucus removing agent plus hypertonic glucose solution (group C) to wash and high-resolution microscopic imaging was made for pretreated specimens of colon polyps and then for pathological examination.The picture quality of the three groups was analyzed. Results The high-resolution microscopic images are 900 for specimens in each polyp, high-quality images in group A were 317 and high quality rate was 35.2%; high-quality images in group B were 493 and high quality rate was 54.8%; and high-quality images in group C were 564 and high quality rate was 62.7%, the difference was statistically significant (P<0.05).Compared with the pathological examination, group A showed simultaneous diagnosis of adenomatous polyps in 9 cases, 8 cases of non-adenomatous polyps, with diagnostic accuracy of 56.7%;group B showed simultaneous diagnosis of adenomatous polyps in 13 cases, non-adenomatous polyps in 7 cases, with diagnostic accuracy of 66.7%; and group C showed simultaneous diagnosis of adenomatous polyps in 12 cases, non-adenomatous polyps in 15 cases, with diagnostic accuracy of 90.0%. The overall accuracy of high-resolution microendoscopy in diagnosis of colon polyps was 71.1%. In Group C, compared with group A, and compared with group B. The difference was statistically significant (P<0.05). Conclusions High-resolution microendoscopy has good imaging results for polyp specimens after pretreatment. It can improve the picture quality and diagnostic accuracy before imaging using saline combined with mucus removing agent and hypertonic glucose solution.
Mark P, Dihua Y, Rebecca R. High-resolution Fiber-optic Microendoscopy for in situ [J]. Cellular Imaging,2011,11(47):2306.
[2]
Pierce M C, Vila P M, Kingsley M J. Accuracy and interrater reliability for the diagnosis of Barrett’s neoplasia among users of a novel,portable high-resolution microendoscope[J]. Diseases of the Esophagus,2014,(27):55-62.
[3]
Pierce M C, Guan Y, Quinn M K,et al. A pilot study of low-cost, high-resolution microendoscopy as a tool for identifying women with cervical precancer[J]. American Association for Cancer,2012,5(11):1273-1279.
[4]
Aslanog lu M. Electrochemical and spectroscopic studies of the interaction of proflavine with DNA[J]. Analytical Sciences,2006,22(3):439-443.
[5]
Peter M. Discrimination of benign and neoplastic mucosa with a high-resolution microendoscope[J]. Ann Surg Oncol,2012,19:3534-3539.
[6]
Regunathan R, Woo J, Pierce M C, et al. Feasibility and preliminary accuracy of high-resolution imaging of the liver and pancreas using FNA compatible microendoscopy(with video)[J]. Gastrointest Endosc,2012,76(2):293-300.
[7]
Neil D. In vivo diagnostic accuracy of high-resolution microendoscopy in differentiating neoplastic from non-neoplastic colorectal polyps: a prospective study[J]. Am J Gastroenterol,2014,109:68-75.
[8]
WANG X, XU W, ZHANG F, et al. Diagnostic value of a new high-resolution microendoscopy for early esophageal cancer[J]. Chin J Dig Endosc,2012,29(1):24-28.
[9]
Chang SS, Shukla1 R. Polydorides et al. High resolution microendoscopy for classification of colorectal polyps[J]. Endoscopy, 2013, 45: 553-559.
Justin S.Applications and advancements in the use of high-resolution microendoscopy for detection of gastrointestinal neoplasia[J]. Clinical Gastroenterology and Hepatology,2014,12(11):1789-1792.
2015, Vol. 26 
