Effects of sodium butyrate on intestinal micro-vascular permeability and blood flow in rats with severe scald injury
TANG Fubo1,2,ZHENG Jinguang1, ZHANG Wenjing3,HU Sen4,and BAI Xiaodong1
1. Department of Burn and Plastic Surgery,3. Department of Infectious Diseases, General Hospital of the Chinese People’s Armed Police Force,Beijing 100039,China; 2. Graduate Term, Logistics University of Chinese People’s Armed Police Force,Tianjin 300309,China; 4. Laboratory of Shock and Organ Dysfunction,Burns Institute,The First Affiliated Hospital of PLA General Hospital,Beijing 100048,China
摘要目的 探讨丁酸钠(sodium butyrate,BTR)对严重烫伤大鼠小肠黏膜血流量和微血管通透性的影响。方法 雄性SD大鼠48只,体重240~260 g,随机分为假烫组、烫伤组和丁酸钠组,每组16只。烫伤组和丁酸钠组采用沸水烫伤背部15 s、腹部8 s,造成50%总体表面积(TBSA)Ⅲ度烫伤;假烫组采用37 ℃温水浸泡相同部位及时间。于烫伤后立即腹腔注射丁酸钠(400 mg/kg)或等体积生理盐水。烫伤后3 h和6 h测定小肠黏膜血流量;检测血浆二胺氧化酶(DAO)活性;取小肠组织检测含水率及微血管通透性。结果 假烫组大鼠小肠黏膜血流量丰富,血浆DAO活性正常,小肠微血管通透性和含水率为正常水平。大鼠严重烫伤后,小肠黏膜血流量迅速降低,DAO活性显著增强,小肠微血管通透性和含水率明显增高。烫伤后3 h,丁酸钠组与烫伤组比较,小肠组织微血管通透性[(4.26±0.98)μg/ml vs (5.53±1.31)μg/ml]、含水率[(63.67±3.35)% vs (74.32±3.74)%]、血浆DAO[(43.76±9.34)U/L vs (73.29±11.34) U/L]均显著降低,小肠黏膜血流量明显升高[(67.21±9.47)BPU vs (55.18±10.48)BPU](均P<0.05)。烫伤后6 h,丁酸钠组与烫伤组比较,小肠微血管通透性[(6.89±1.12)μg/ml vs (8.92±1.69) μg/ml]、含水率[(68.45±4.52)% vs (80.76±3.94)%]、血浆DAO[(47.59±10.71)U/L vs (89.87±11.93) U/L]均明显降低,小肠黏膜血流量明显升高[(47.77±8.93)BPU vs (25.64±7.42)BPU](均P<0.05)。结论 丁酸钠能增加严重烫伤大鼠小肠黏膜血流量,降低小肠微血管内皮通透性和组织水肿,对小肠组织具有保护作用。
Abstract：Objective To investigate the small intestine micro-vascular permeability and blood flow protective effects of sodium butyrate on rats following 50%TBSA full-thickness burns. Methods 48 SD rats, weighing 240-260 g, were randomly divided into three groups: sham group, scald group, scald + BTR group. Rats in scald group and scald + BTR group were subjected to 50% TBSA third-degree burns by immersing the back for 15 seconds and the abdomen for 8 seconds in boiling water. The sham group was immersed in 37 ℃ water instead. And then 1ml BTR (400 mg/kg) or 1ml normal saline was intraperitoneally injected, respectively. Blood flow in small intestine was measured by Doppler 3 and 6 hours after injury. Blood was drawn from the heart at 3 and 6 hours after injury for measurement of serum diamine oxidase (DAO); the small intestine tissues were harvested for the measurement of micro-vascular permeability and the changes in tissue water content. Results Compared with scald group, the activity of DAO [(43.76±9.34)U/L vs (73.29±11.34) U/L], the micro-vascular permeability [(4.26±0.98)μg/ml vs (5.53±1.31) μg/ml] and the rate of tissue water [(63.67±3.35)% vs (74.32±3.74)%] in scald + BTR group were significantly lower at 3 hours after scald injury (all P<0.05). In addition, BTR was shown to significantly increase the IMBF level [(67.21±9.47)BPU vs (55.18±10.48)BPU] induced by scald injury at 3 hours (P<0.05). The result at 6 hours after scald injury was similar to 3 hours. Conclusions Sodium butyrate has significant protective effects on small intestinal micro-vascular permeability and blood flow in rats with severe scald injury.
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2016, Vol. 27 
