1.Digestive System Department,Shaanxi Provincial Crops Hospital of Chinese People’s Armed Police Force, Xi’an 710054, China; 2.Health Division, Rear-service Department of Chinese People’s Armed Police Force, Beijing 100089,China; 3.Department of Traditional Chinese Medicine,First Affiliated Hospital,Xi’an Jiaotong University,Xi’an 710061, China
Abstract：Objective To investigate the effect of FRCS on expression of bone ER-αin rats with ovariectomy so as to provide evidence for the theory that the kidney dominates bones.Methods Four-month-old Sprague Dawley female rats were randomly divided into sham operated group, model control group, estrogen treatment group and FRCS treatment group. Only the fat around the ovary was removed in sham operated group, while ovaries were removed in other groups. Four weeks after operation, estrogen and FRCS were administered to rats of treatment groups respectively, and the treatment lasted four weeks. Then, the bone morphology was evaluated by HE stain, the serum level of E2 was detected by radio-immunity, the protein expression of ER-α in cells of the bone tissue surface was investigated by immunohistochemical method.Results Compared with sham operated group, the bone trabecula in model control group was sparce and disorderly, the trabecular interspace was enlarged more obviously, and the serum level of E2 decreased(P<0.01). Compared with model control group, the serum level of E2 in estrogen treatment group and FRCS treatment group increased(P<0.01)and the bone brabecula was more continual and aligned in better order, the uterus index changed as serum E2 did. Compared with sham operated group, the expression of ER-α decreased in the articular cartilage, epiphyseal plate, bone trabecula and bone marrow(All P<0.05)in model control group. Compared with model control group, the expression of ER-α increased in estrogen treatment group and FRCS treatment group(All P<0.05).Conclusions The mechanism by which FRCS can prevent and cure postmenopausal osteoporosis may lie in the ability of FRCS to modulate the expression of bone ER-α.
Sahin Ersoy G,Giray B,Subas S, et al. Interpregnancy interval as a risk factor for postmenopausal osteoporosis[J]. Maturitas, 2015,82(2):236-240.
[5]
Imai Y,Kondoh S,Kouzmenko A,et al. Minireview: osteoprotective action of estrogens is mediated by osteoclastic estrogen receptor-α[J]. Mol Endocrinol, 2010, 24(5): 877-885.
[6]
Shoukry A, Shalaby S M, Etewa R L, et al. Association of estrogen receptor β and estrogen-related receptor α gene polymorphisms with bone mineral density in postmenopausal women[J]. Mol Cell Biochem, 2015, 405(1-2): 23-31.
[7]
Mitra S,Desai M,Khatkhatay M I. Association of estrogen receptor α gene polymorphisms with bone mineral density in postmenopausal Indian women [J]. Mol Genet Metab, 2006, 87(1): 80-87.
[8]
Gambacciani M,Levancini M. Management of postmenopausal osteoporosis and the prevention of fractures [J]. Panminerva Med, 2014, 56(2): 115-131.
[9]
Clarkson T B, Meléndez G C, Appt S E. Timing hypothesis for postmenopausal hormone therapy: its origin, current status, and future [J]. Menopause, 2013, 20(3): 342-353.
[10]
Kulak Júnior J,Kulak C A,Taylor H S. SERMs in the prevention and treatment of postmenopausal osteoporosis: an update [J]. Arq Bras Endocrinol Metabol, 2010, 54(2): 200-205.
[11]
Komm B S,Mirkin S. An overview of current and emerging SERMs [J]. J Steroid Biochem Mol Biol, 2014,143:207-222.
[12]
Craig Jordan V, McDaniel R, Agboke F, et al. The evolution of nonsteroidal antiestrogens to become selective estrogen receptor modulators [J]. Steroids, 2014,90:3-12.
Rajalin A, Pollock H, Aarnisalo P. ERR alpha regulates osteoblastic and adipogenic differentiation of mouse bone marrow mesenchymal stem cells [J]. Biochem Biophys Res Commun, 2010, 396(2): 477-482.
[17]
Manolagas S C, O’Brien C A, Almeida M. The role of estrogen and androgen receptors in bone health and disease [J]. Nat Rev Endocrinol, 2013, 9(12): 699-712.
[18]
Wang J, Shang F, Mei Q, et al. NO-donating genistein prodrug alleviates bone loss in ovariectomised rats [J]. Swiss Medical Wkly, 2008, 438(41): 602-607.
2017, Vol. 28 
